Assembly of the protoplasm of Codium fragile (Bryopsidales, Chlorophyta) into new protoplasts

doi:10.1111/j.1744-7909.2008.00671.x

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	Assembly of the protoplasm of Codium fragile (Bryopsidales, Chlorophyta) into new protoplasts
	Li, Demao 1,3,4; Lue, Fang 1,4; Wang, Guangce 1,2; Zhou, Baicheng 1
	2008-06-01
发表期刊	JOURNAL OF INTEGRATIVE PLANT BIOLOGY
ISSN	1672-9072
卷号	50 期号:6 页码:752-760
文章类型	Article
摘要	The cell organelles of the coenocytic alga Codium fragile (Sur.) Hariot aggregated rapidly and protoplasts were formed when its protoplasm was extruded out in seawater. Continuous observation showed that there were long and gelatinous threads connecting the cell organelles. The threads contracted, and thus the cell organelles aggregated into protoplasmic masses. The enzyme digestion experiments and Coomassie Brilliant Blue and Anthrone stainings showed that the long and gelatinous threads involved in the formation of the protoplasts might include protein and saccharides as structure components. Nile Red staining indicated that the protoplast primary envelope was non-lipid at first, and then lipid materials integrated into its surface gradually. The fluorescent brightener staining indicated that the cell wall did not regenerate in the newly formed protoplasts and they all disintegrated within 72 h after formation. Transmission electron microscopy of the cell wall of wild C. fragile showed electron-dense material embedded in the whole cell wall at regular intervals. The experiments indicated that C. fragile would be a suitable model alga for studying the formation of protoplasts.; The cell organelles of the coenocytic alga Codium fragile (Sur.) Hariot aggregated rapidly and protoplasts were formed when its protoplasm was extruded out in seawater. Continuous observation showed that there were long and gelatinous threads connecting the cell organelles. The threads contracted, and thus the cell organelles aggregated into protoplasmic masses. The enzyme digestion experiments and Coomassie Brilliant Blue and Anthrone stainings showed that the long and gelatinous threads involved in the formation of the protoplasts might include protein and saccharides as structure components. Nile Red staining indicated that the protoplast primary envelope was non-lipid at first, and then lipid materials integrated into its surface gradually. The fluorescent brightener staining indicated that the cell wall did not regenerate in the newly formed protoplasts and they all disintegrated within 72 h after formation. Transmission electron microscopy of the cell wall of wild C. fragile showed electron-dense material embedded in the whole cell wall at regular intervals. The experiments indicated that C. fragile would be a suitable model alga for studying the formation of protoplasts.
关键词	Codium Fragile Protoplasm Protoplast Cell Wall
DOI	10.1111/j.1744-7909.2008.00671.x
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000256532500014
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/6286
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Tainjin Univ Sci & Technol, Coll Marine Sci & Engn, Tianjin 300457, Peoples R China 3.Liaocheng Univ, Coll Agr, Dept Food Sci, Liaocheng 252059, Peoples R China 4.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Li, Demao,Lue, Fang,Wang, Guangce,et al. Assembly of the protoplasm of Codium fragile (Bryopsidales, Chlorophyta) into new protoplasts[J]. JOURNAL OF INTEGRATIVE PLANT BIOLOGY,2008,50(6):752-760.
APA	Li, Demao,Lue, Fang,Wang, Guangce,&Zhou, Baicheng.(2008).Assembly of the protoplasm of Codium fragile (Bryopsidales, Chlorophyta) into new protoplasts.JOURNAL OF INTEGRATIVE PLANT BIOLOGY,50(6),752-760.
MLA	Li, Demao,et al."Assembly of the protoplasm of Codium fragile (Bryopsidales, Chlorophyta) into new protoplasts".JOURNAL OF INTEGRATIVE PLANT BIOLOGY 50.6(2008):752-760.

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