Combinational biosynthesis of a fluorescent cyanobacterial holo-alpha-allophycocyanin in Escherichia coli

doi:10.1007/s10529-008-9644-2

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	Combinational biosynthesis of a fluorescent cyanobacterial holo-alpha-allophycocyanin in Escherichia coli
	Yang, Yu 1,2; Ge, Baosheng 3; Guan, Xiangyu 4; Zhang, Weijie 1,2; Qin, Song 1
	2008-06-01
发表期刊	BIOTECHNOLOGY LETTERS
ISSN	0141-5492
卷号	30 期号:6 页码:1001-1004
文章类型	Article
摘要	Allophycocyanin ( APC) is a phycobiliprotein with various biological and pharmacological properties. An expression vector containing five essential genes in charge of biosynthesis of cyanobacterial APC holo-alpha subunit ( holo- ApcA) was constructed, resulting in over- expression of a fluorescent holo- ApcA in E. coli. After being cultured for 16 h, the dry cell density reached 22.5 gl(-1), and the expression of holo- HT- ApcA was up to 1 gl(-1) broth. The recombinant protein showed similar spectral features to native APC.; Allophycocyanin ( APC) is a phycobiliprotein with various biological and pharmacological properties. An expression vector containing five essential genes in charge of biosynthesis of cyanobacterial APC holo-alpha subunit ( holo- ApcA) was constructed, resulting in over- expression of a fluorescent holo- ApcA in E. coli. After being cultured for 16 h, the dry cell density reached 22.5 gl(-1), and the expression of holo- HT- ApcA was up to 1 gl(-1) broth. The recombinant protein showed similar spectral features to native APC.
关键词	Allophycocyanin Escherichia Coli Fluorescence Recombinant Protein
学科领域	Biotechnology & Applied Microbiology
DOI	10.1007/s10529-008-9644-2
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000255190300005
引用统计	被引频次：12[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/5702
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Shandong, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.China Univ Petr, Ctr Biotechnol & Bioengn, Qingdao 266555, Peoples R China 4.Ocean Univ China, Qingdao 266003, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Yang, Yu,Ge, Baosheng,Guan, Xiangyu,et al. Combinational biosynthesis of a fluorescent cyanobacterial holo-alpha-allophycocyanin in Escherichia coli[J]. BIOTECHNOLOGY LETTERS,2008,30(6):1001-1004.
APA	Yang, Yu,Ge, Baosheng,Guan, Xiangyu,Zhang, Weijie,&Qin, Song.(2008).Combinational biosynthesis of a fluorescent cyanobacterial holo-alpha-allophycocyanin in Escherichia coli.BIOTECHNOLOGY LETTERS,30(6),1001-1004.
MLA	Yang, Yu,et al."Combinational biosynthesis of a fluorescent cyanobacterial holo-alpha-allophycocyanin in Escherichia coli".BIOTECHNOLOGY LETTERS 30.6(2008):1001-1004.

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