Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
DNA barcoding discriminates Pampus minor (Liu et al, 1998) from Pampus species | |
Guo Enmian2; Liu Yuan1; Liu Jing1; Cui Zhaoxia1 | |
2010-11-01 | |
发表期刊 | CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY |
卷号 | 28期号:6页码:1266-1274 |
文章类型 | Article |
摘要 | Although Pampus minor has been classified as a new species, it still remains controversial Was used a DNA barcoding technique based on homologous sequence analysis of the 16S and COI genes to clarify the confusion over the identification of this species Among 12 individuals whose genetic distance was 0 002, two haplotypes were found According to the 16S sequences, the genetic distances ranged from 0 121 to 0 133 between P minor and other Pampus species Although the same the genetic distance between the two P minor haplotypes was generated using COI sequences, the haplotype of Pm22-23, Pm28, and Pm32-33 was the same as that of Pci EF607462 and EF607466, while the haplotype of Pm24-27 and Pm29-31 was the same as that of Pci EF607461 and EF607463-65 In addition the genetic distance ranged only from 0 002 to 0 005 between P minor and Pa EF607460 and EF607458 Apart from this, the mterspecies genetic distances varied from 0 135 to 0 143 between P minor and other Pampus species according to the COI sequences Phylogenetic trees using combined 16S and COI data, strongly support the viewpoint that all the P minor individuals form one clade that is in a sister position to Pampus sp individuals (EU357803, FJ434342-FJ434343, and FJ652423-FJ652427) |
关键词 | Pampus Minor Coi 16s Dna Barcoding |
DOI | 10.1007/s00343-010-9917-1 |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000285279700022 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qdio.ac.cn/handle/337002/32736 |
专题 | 实验海洋生物学重点实验室 |
作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Qingdao Agr Univ, Qingdao 266109, Peoples R China |
推荐引用方式 GB/T 7714 | Guo Enmian,Liu Yuan,Liu Jing,et al. DNA barcoding discriminates Pampus minor (Liu et al, 1998) from Pampus species[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2010,28(6):1266-1274. |
APA | Guo Enmian,Liu Yuan,Liu Jing,&Cui Zhaoxia.(2010).DNA barcoding discriminates Pampus minor (Liu et al, 1998) from Pampus species.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,28(6),1266-1274. |
MLA | Guo Enmian,et al."DNA barcoding discriminates Pampus minor (Liu et al, 1998) from Pampus species".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 28.6(2010):1266-1274. |
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