Effect of lanthanum on expression of recombinant allophycocyanin gene in Pichia pastotis

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	Effect of lanthanum on expression of recombinant allophycocyanin gene in Pichia pastotis
	Ren, YH ; Ge, BS ; Jin, HZ ; Tang, ZH ; Guo, CH ; Qin, S
	2005-08-01
发表期刊	JOURNAL OF RARE EARTHS
ISSN	1002-0721
卷号	23 期号:4 页码:491-495
文章类型	Article
摘要	The enhancing effect of lanthanum on gene expression of recombinant allophycocyanin (rAPC), a potential antitumor medicine, in Pichia pastoris was studied. PCR and sequence analysis were used in order to prove whether the APC gene had integrated into the yeast genome. The expression level of the recombinant allophycocyanin (rAPC) in BMMY medium containing LaCl3 was detected by ELISA method. The recombinant allophycocyanin was determined by Western blot. The results show that the recombinant Pichia pastoris chromosome contained allophycocyanin gene. Expression efficiency of rAPC gene in Pichia pastoris was promoted by proper LaCl3 concentration like 2, 5, 10 mmol (.) L-1, among which 5 mmol (.) L-1 was the most effective. The highest expression yield of rAPC in the BMMY medium containing 5 mmol (.) L-1 LaCl3 was 4.4 mg (.) L-1 at 48 h, that was increased by 110% compared with 2.1 mg (.) L-1 of control, in the meantime, the optimum culture time is shortened from 72 to 48 h. The result of western blot analysis indicates that the rAPC consisted of two kinds of subunits with molecular weight of 19 and 21 kDa respectively.; The enhancing effect of lanthanum on gene expression of recombinant allophycocyanin (rAPC), a potential antitumor medicine, in Pichia pastoris was studied. PCR and sequence analysis were used in order to prove whether the APC gene had integrated into the yeast genome. The expression level of the recombinant allophycocyanin (rAPC) in BMMY medium containing LaCl3 was detected by ELISA method. The recombinant allophycocyanin was determined by Western blot. The results show that the recombinant Pichia pastoris chromosome contained allophycocyanin gene. Expression efficiency of rAPC gene in Pichia pastoris was promoted by proper LaCl3 concentration like 2, 5, 10 mmol (.) L-1, among which 5 mmol (.) L-1 was the most effective. The highest expression yield of rAPC in the BMMY medium containing 5 mmol (.) L-1 LaCl3 was 4.4 mg (.) L-1 at 48 h, that was increased by 110% compared with 2.1 mg (.) L-1 of control, in the meantime, the optimum culture time is shortened from 72 to 48 h. The result of western blot analysis indicates that the rAPC consisted of two kinds of subunits with molecular weight of 19 and 21 kDa respectively.
关键词	Lanthanum Chloride Recombinant Allophycoeyanin Expression Pichia Pastoris Rare Earths
学科领域	Chemistry, Applied
收录类别	SCI
语种	英语
WOS记录号	WOS:000232021000023
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/3122
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Yantai Univ, Dept Biochem, Yantai 264005, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Ren, YH,Ge, BS,Jin, HZ,et al. Effect of lanthanum on expression of recombinant allophycocyanin gene in Pichia pastotis[J]. JOURNAL OF RARE EARTHS,2005,23(4):491-495.
APA	Ren, YH,Ge, BS,Jin, HZ,Tang, ZH,Guo, CH,&Qin, S.(2005).Effect of lanthanum on expression of recombinant allophycocyanin gene in Pichia pastotis.JOURNAL OF RARE EARTHS,23(4),491-495.
MLA	Ren, YH,et al."Effect of lanthanum on expression of recombinant allophycocyanin gene in Pichia pastotis".JOURNAL OF RARE EARTHS 23.4(2005):491-495.

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