Suspension culture of gametophytes of transgenic kelp in a photobioreactor

doi:10.1007/s10529-005-8098-z

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	Suspension culture of gametophytes of transgenic kelp in a photobioreactor
	Gao, JT ; Zhang, YC ; Wang, HH ; Qin, S
	2005-07-01
发表期刊	BIOTECHNOLOGY LETTERS
ISSN	0141-5492
卷号	27 期号:14 页码:1025-1028
文章类型	Article
摘要	Transgenic Laminaria japonica gametophytes producing a recombinant tissue-type plasminogen activator (rtPA) protein, which is an effective third-generation thrombolytic agent for acute myocardial infarction (AMI), were cultured in an illuminated bubble column bioreactor. A maximum final dry cell weight of 1120 mg l(-1) was obtained in batch culture with an initial dry cell weight of 126 mg l(-1) and with aeration rate of 1.2 l air min(-1) l(-1) culture, nitrate at 1.5 mM and phosphate at 0.17 mM. The yield of rtPA was 56 mu g g(-1) dry cell wt. This is the first report regarding cultivation of a transgenic macroalga in a bioreactor.; Transgenic Laminaria japonica gametophytes producing a recombinant tissue-type plasminogen activator (rtPA) protein, which is an effective third-generation thrombolytic agent for acute myocardial infarction (AMI), were cultured in an illuminated bubble column bioreactor. A maximum final dry cell weight of 1120 mg l(-1) was obtained in batch culture with an initial dry cell weight of 126 mg l(-1) and with aeration rate of 1.2 l air min(-1) l(-1) culture, nitrate at 1.5 mM and phosphate at 0.17 mM. The yield of rtPA was 56 mu g g(-1) dry cell wt. This is the first report regarding cultivation of a transgenic macroalga in a bioreactor.
关键词	Bioreactor Gametophytes Macroalga Plasminogen Activator Protein Seaweed Transgenic Kelp
学科领域	Biotechnology & Applied Microbiology
DOI	10.1007/s10529-005-8098-z
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000231471000011
引用统计	被引频次：13[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/3092
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Gao, JT,Zhang, YC,Wang, HH,et al. Suspension culture of gametophytes of transgenic kelp in a photobioreactor[J]. BIOTECHNOLOGY LETTERS,2005,27(14):1025-1028.
APA	Gao, JT,Zhang, YC,Wang, HH,&Qin, S.(2005).Suspension culture of gametophytes of transgenic kelp in a photobioreactor.BIOTECHNOLOGY LETTERS,27(14),1025-1028.
MLA	Gao, JT,et al."Suspension culture of gametophytes of transgenic kelp in a photobioreactor".BIOTECHNOLOGY LETTERS 27.14(2005):1025-1028.

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