Characterization of a new lectin involved in the protoplast regeneration of Bryopsis hypnoides

doi:10.1007/s00343-009-9157-4

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	Characterization of a new lectin involved in the protoplast regeneration of Bryopsis hypnoides
	Niu Jianfeng 1; Wang Guangce 1,2; Lue Fang 1,3; Zhou Baicheng 1; Peng Guang 1
	2009-08-01
发表期刊	CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN	0254-4059
卷号	27 期号:3 页码:502-512
文章类型	Article
摘要	A group of coenocytic marine algae differs from higher plants, whose totipotency depends on an intact cell (or protoplast). Instead, this alga is able to aggregate its extruded protoplasm in sea water and generate new mature individuals. It is thought that lectins play a key role in the aggregation process. We purified a lectin associated with the aggregation of cell organelles in Bryopsis hypnoides. The lectin was ca. 27 kDa with a pI between pH 5 and pH 6. The absence of carbohydrate suggested that the lectin was not a glycoprotein. The hemagglutinating activity (HA) of the lectin was not dependent on the. presence of divalent cations and was inhibited by N-Acetylgalactosamine, N-Acetylglucosamine, and the glycoprotein bovine submaxillary mucin. The lectin preferentially agglutinated Gram-negative bacterium. The HA of this lectin was stable between pH 4 to pH 10. Cell organelles outside the cytoplasm were agglutinated by the addition of lectin solution (0.5 mg ml(-1)). Our results suggest that the regeneration of B. hypnoides is mediated by this lectin. We also demonstrated that the formation of cell organelle aggregates was inhibited by nigericin in natural seawater (pH 8.0). Given that nigericin dissipates proton gradients across the membrane, we hypothesize that the aggregation of cell organelles was proton-gradient dependent.; A group of coenocytic marine algae differs from higher plants, whose totipotency depends on an intact cell (or protoplast). Instead, this alga is able to aggregate its extruded protoplasm in sea water and generate new mature individuals. It is thought that lectins play a key role in the aggregation process. We purified a lectin associated with the aggregation of cell organelles in Bryopsis hypnoides. The lectin was ca. 27 kDa with a pI between pH 5 and pH 6. The absence of carbohydrate suggested that the lectin was not a glycoprotein. The hemagglutinating activity (HA) of the lectin was not dependent on the. presence of divalent cations and was inhibited by N-Acetylgalactosamine, N-Acetylglucosamine, and the glycoprotein bovine submaxillary mucin. The lectin preferentially agglutinated Gram-negative bacterium. The HA of this lectin was stable between pH 4 to pH 10. Cell organelles outside the cytoplasm were agglutinated by the addition of lectin solution (0.5 mg ml(-1)). Our results suggest that the regeneration of B. hypnoides is mediated by this lectin. We also demonstrated that the formation of cell organelle aggregates was inhibited by nigericin in natural seawater (pH 8.0). Given that nigericin dissipates proton gradients across the membrane, we hypothesize that the aggregation of cell organelles was proton-gradient dependent.
关键词	Bryopsis Hypnoides Lectin Hemagglutinating Cell Organelle Aggregation Regeneration
学科领域	Limnology ; Oceanography
DOI	10.1007/s00343-009-9157-4
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000270742000011
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/2996
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Tianjin Univ Sci & Technol, Coll Marine Sci & Engn, Tianjin 300457, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Niu Jianfeng,Wang Guangce,Lue Fang,et al. Characterization of a new lectin involved in the protoplast regeneration of Bryopsis hypnoides[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2009,27(3):502-512.
APA	Niu Jianfeng,Wang Guangce,Lue Fang,Zhou Baicheng,&Peng Guang.(2009).Characterization of a new lectin involved in the protoplast regeneration of Bryopsis hypnoides.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,27(3),502-512.
MLA	Niu Jianfeng,et al."Characterization of a new lectin involved in the protoplast regeneration of Bryopsis hypnoides".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 27.3(2009):502-512.

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