cDNA cloning and gene expression pattern following bacterial challenge of peroxinectin in Chinese shrimp Fenneropenaeus chinensis

doi:10.1007/s11033-009-9453-2

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	cDNA cloning and gene expression pattern following bacterial challenge of peroxinectin in Chinese shrimp Fenneropenaeus chinensis
	Dong, Bo; Liu, Fengsong; Gao, Hongwei; Wang, Bing; Xiang, Jianhai
	2009-11-01
发表期刊	MOLECULAR BIOLOGY REPORTS
ISSN	0301-4851
卷号	36 期号:8 页码:2333-2339
文章类型	Article
摘要	Peroxinectin, a cell-adhesive hemoperoxidase that binds superoxide dismutase and mediates blood cells adhesion and migration in invertebrate, is believed to play an important role in cellular immune reaction. In this study, we reported a new peroxinectin gene homologue from Chinese shrimp Fenneropenaeus chinensis. Based on expressed sequence tags (ESTs) of haemocyte cDNA library, we cloned a 2,611 bps full-length cDNA of peroxinectin gene homologue encoded 801 amino acids. Motif scanning of the predicted polypeptide revealed a peroxidase domain and an integrin binding motif (Lys-Gly-Asp, KGD). Peroxinectin gene expressed constitutively in haemocyte as determined by quantitative real-time RT-PCR, the expression level varied following bacterial challenge. These findings suggested that peroxinectin expression is susceptible to exterior stimulus and maintains at a high expression level during bacterial infection.; Peroxinectin, a cell-adhesive hemoperoxidase that binds superoxide dismutase and mediates blood cells adhesion and migration in invertebrate, is believed to play an important role in cellular immune reaction. In this study, we reported a new peroxinectin gene homologue from Chinese shrimp Fenneropenaeus chinensis. Based on expressed sequence tags (ESTs) of haemocyte cDNA library, we cloned a 2,611 bps full-length cDNA of peroxinectin gene homologue encoded 801 amino acids. Motif scanning of the predicted polypeptide revealed a peroxidase domain and an integrin binding motif (Lys-Gly-Asp, KGD). Peroxinectin gene expressed constitutively in haemocyte as determined by quantitative real-time RT-PCR, the expression level varied following bacterial challenge. These findings suggested that peroxinectin expression is susceptible to exterior stimulus and maintains at a high expression level during bacterial infection.
关键词	Cell Adhesion Gene Peroxinectin Expression Pattern Real-time Rt-pcr Fenneropenaeus Chinensis
学科领域	Biochemistry & Molecular Biology
DOI	10.1007/s11033-009-9453-2
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000270984600039
引用统计	被引频次：18[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/2898
专题	实验海洋生物学重点实验室
作者单位	Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Dong, Bo,Liu, Fengsong,Gao, Hongwei,et al. cDNA cloning and gene expression pattern following bacterial challenge of peroxinectin in Chinese shrimp Fenneropenaeus chinensis[J]. MOLECULAR BIOLOGY REPORTS,2009,36(8):2333-2339.
APA	Dong, Bo,Liu, Fengsong,Gao, Hongwei,Wang, Bing,&Xiang, Jianhai.(2009).cDNA cloning and gene expression pattern following bacterial challenge of peroxinectin in Chinese shrimp Fenneropenaeus chinensis.MOLECULAR BIOLOGY REPORTS,36(8),2333-2339.
MLA	Dong, Bo,et al."cDNA cloning and gene expression pattern following bacterial challenge of peroxinectin in Chinese shrimp Fenneropenaeus chinensis".MOLECULAR BIOLOGY REPORTS 36.8(2009):2333-2339.

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