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| Comparative transcriptomic analysis provides insights into the molecular basis of the metamorphosis and nutrition metabolism change from zoeae to megalopae in Eriocheir sinensis | |
Li, Yingdong1; Hui, Min1; Cui, Zhaoxia1,2; Liu, Yuan1; Song, Chengwen1,3 ; Shi, Guohui1,3
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| 2015-03-01 | |
| 发表期刊 | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
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| 卷号 | 13页码:1-9 |
| 文章类型 | Article |
| 摘要 | Within the larval period of Eriocheir sinensis, there is pronounced morphological changes upon the molt from the fifth zoeae (Z5) to megalopae (M), and low survival rate exists during this transition, which is typical in crab species. RNA sequencing was applied to 25 and M of E. sinensis, resulting in the discovery of 19,186 unigenes and 652 differentially expressed genes (DEGs, 3.40% of the unigenes). The important metabolic pathways that might play roles in the larval development of E sinensis from Z5 to M were detected to be 'Xenobiotics Biodegradation and Metabolism (8.16%)', 'Metabolism of Cofactors and Vitamins (6.70%)', 'Lipid Metabolism (6.36%)', and 'Amino Acid Metabolism (6.28%)'. Further, 19 DEGs possibly contributing to the morphological and sensory capability changes of the larvae were identified, like multiple copies of cuticle protein genes, retinaldehyde-binding protein 1 (RLBP1), envelope protein (Envelope) and hormone-related gene ecdysteroid-regulated 16 kDa protein (ESR16). Moreover, 62 DEGs were identified to be related to carbohydrate, lipid and protein digestion and metabolism, such as glucose dehydrogenases (GDHs), lipases (LIPS) and serine proteases (SPs). Among these DEGs, more genes related to the substance metabolism were found up-regulated in Z5 than M. suggesting that more energy might be essential to be released for 25 to complete the transition into M. Characterization of the crucial DEGs by real-time quantitative PCR re-conformed their expression pattern. This study provides the first genome-wide transcriptomic analysis of E. sinensis 25 and M for studying the molecular basis of the larvae metamorphosis and nutrition metabolism. (C) 2014 Elsevier Inc. All rights reserved. |
| 关键词 | Larval Development Metamorphosis Mitten Crab Nutrition Metabolism Transcriptomics |
| DOI | 10.1016/j.cbd.2014.10.002 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000351032100001 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/25405 |
| 专题 | 海洋生态与环境科学重点实验室 实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, EMBL, Qingdao 266071, Peoples R China 2.Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Li, Yingdong,Hui, Min,Cui, Zhaoxia,et al. Comparative transcriptomic analysis provides insights into the molecular basis of the metamorphosis and nutrition metabolism change from zoeae to megalopae in Eriocheir sinensis[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,2015,13:1-9. |
| APA | Li, Yingdong,Hui, Min,Cui, Zhaoxia,Liu, Yuan,Song, Chengwen,&Shi, Guohui.(2015).Comparative transcriptomic analysis provides insights into the molecular basis of the metamorphosis and nutrition metabolism change from zoeae to megalopae in Eriocheir sinensis.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,13,1-9. |
| MLA | Li, Yingdong,et al."Comparative transcriptomic analysis provides insights into the molecular basis of the metamorphosis and nutrition metabolism change from zoeae to megalopae in Eriocheir sinensis".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS 13(2015):1-9. |
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