The immunomodulation of inducible hydrogen sulfide in Pacific oyster Crassostrea gigas

doi:10.1016/j.dci.2014.03.011

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	The immunomodulation of inducible hydrogen sulfide in Pacific oyster Crassostrea gigas
	Sun, Zhibin 1; Wang, Lingling 1; Zhang, Tao1,2 ; Zhou, Zhi 1; Jiang, Qiufen 1,2; Yi, Qilin 1,2; Yang, Chuanyan 1; Qiu, Limei 1; Song, Linsheng 1; Wang, LL (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China.
	2014-10-01
发表期刊	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷号	46 期号:2 页码:530-536
文章类型	Article
摘要	Hydrogen sulfide (H2S) is an important gasotransmitter, which plays indispensable roles in cardiovascular, nervous and immune systems of vertebrates. However, the information about the immunomodulaton of H2S in invertebrates is still very limited. In the present study, the temporal expression profile of cystathionine gamma lyase in oyster Crassostrea gigas (CgCSE) was investigated after the oysters were stimulated by lipopolysaccharide. The expression levels of CgCSE mRNA transcripts in hemocytes increased significantly at 12 h (1.31-fold of the PBS group, P < 0.05) after LPS stimulation. The immunomodulation of inducible H2S in oyster was examined by monitoring the alterations of both cellular and humoral immune parameters in response to the stimulations of LPS, LPS + Na2S and LPS + propargylglycine (PAG). The total hemocyte counts (THC) and hemolymph PO activity increased significantly after LPS stimulation, and the increase could be further enhanced by adding PAG, while inhibited by appending Na2S. The phagocytosis activity of hemocytes was also increased firstly after LPS treatment, and the increase was enhanced by adding Na2S but inhibited after appending PAG. The anti-bacterial activity in hemolymph increased at 3 h post LPS treatment, and then decreased after adding PAG. The total SOD activity of hemolymph was also elevated at 6 h post LPS treatment, and the elevated activity was depressed by adding Na2S. These results collectively indicated that H2S might play crucial roles in the immune response of oyster via modulating the turnover and phagocytosis of hemocytes, and regulating the anti-bacterial activity and proPO activation in the hemolymph. (C) 2014 Elsevier Ltd. All rights reserved.
关键词	Crassostrea Gigas Hydrogen Sulfide (H2s) Cystathionine Gamma Lyase (Cse) Immunity Lps Propargylglycine (Pag)
学科领域	Immunology ; Zoology
DOI	10.1016/j.dci.2014.03.011
收录类别	SCI
语种	英语
WOS记录号	WOS:000340216600044
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/24179
专题	实验海洋生物学重点实验室海洋生态与环境科学重点实验室
通讯作者	Wang, LL (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China.
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Sun, Zhibin,Wang, Lingling,Zhang, Tao,et al. The immunomodulation of inducible hydrogen sulfide in Pacific oyster Crassostrea gigas[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2014,46(2):530-536.
APA	Sun, Zhibin.,Wang, Lingling.,Zhang, Tao.,Zhou, Zhi.,Jiang, Qiufen.,...&Wang, LL .(2014).The immunomodulation of inducible hydrogen sulfide in Pacific oyster Crassostrea gigas.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,46(2),530-536.
MLA	Sun, Zhibin,et al."The immunomodulation of inducible hydrogen sulfide in Pacific oyster Crassostrea gigas".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 46.2(2014):530-536.

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