Antioxidative capacity and enzyme activity in Haematococcus pluvialis cells exposed to superoxide free radicals

IOCAS-IR

	Antioxidative capacity and enzyme activity in Haematococcus pluvialis cells exposed to superoxide free radicals
	Liu JG(刘建国); Zhang XL(张晓丽); Sun YH(孙延红); Lin W(林伟)
发表期刊	Chinese Journal of Oceanology and Limnology
ISSN	0254-4059
	2010-02-15
出版年	2010
卷号	v.28 期号:01 页码:3-11
文献类型	CNKI期刊论文
摘要	The antioxidative capacity of astaxanthin and enzyme activity of reactive oxygen eliminating enzymes such as superoxide dismutase (SOD),peroxidase (POD),catalase (CAT) and ascorbate peroxidase (APX) were studied in three cell types of Haematococcus pluvialis exposed to high concentrations of a superoxide anion radical (O2ˉ).The results show that defensive enzymes and astaxanthin-related mechanisms were both active in H.pluvialis during exposure to reactive oxygen species (ROS) such as Oˉ2.Astaxanthin reacted with ROS much faster than did the protective enzymes,and had the strongest antioxidative capacity to protect against lipid peroxidation.The defensive mechanisms varied significantly between the three cell types and were related to the level of astaxanthin that had accumulated in those cells.Astaxanthin-enriched red cells had the strongest antioxidative capacity,followed by brown cells,and astaxanthin-deficient green cells.Although there was no significant increase in expression of protective enzymes,the malondialdehyde (MDA) content in red cells was sustained at a low level because of the antioxidative effect of astaxanthin,which quenched Oˉ2 before the protective enzymes could act.In green cells,astaxanthin is very low or absent;therefore,scavenging of ROS is inevitably reliant on antioxidative enzymes.Accordingly,in green cells,these enzymes play the leading role in scavenging ROS,and the expression of these enzymes is rapidly increased to reduce excessive ROS.However,because ROS were constantly increased in this study,the enhance enzyme activity in the green cells was not able to repair the ROS damage,leading to elevated MDA content.Of the four defensive enzymes measured in astaxanthin-deficient green cells,SOD eliminates Oˉ2,POD eliminates H2O2,which is a by-product of SOD activity,and APX and CAT are then initiated to scavenge excessive ROS.
关键词	astaxanthin Haematococcus pluvialis lipid peroxidation reactive oxygen species (ROS)
CNKI专辑号	A;
CNKI专辑名称	基础科学;
CNKI专题号	A006
CNKI专题名称	生物学;
分类号	Q946
收录类别	中科院核心
语种	英文;
资助项目(基金）	Supported by the National High Technology Research and Development Program of China (863 Program) (No. 2008AA09Z403) ; the Special Project for Marine Public Welfare Industry (No.200705010) ; the National Natural Science Foundation of China (No. 30771638)
文献类型	CNKI期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/192245
专题	中国科学院海洋研究所
作者单位	1.ResearchandDevelopmentCenterofMarineBiotechnology,InstituteofOceanology,ChineseAcademyofSciences 2.GraduateUniversityofChineseAcademyofSciences
推荐引用方式 GB/T 7714	Liu JG,Zhang XL,Sun YH,et al. Antioxidative capacity and enzyme activity in Haematococcus pluvialis cells exposed to superoxide free radicals. 2010.