DNA barcoding of Antarctic marine zooplankton for species identification and recognition

IOCAS-IR

	DNA barcoding of Antarctic marine zooplankton for species identification and recognition
	CHENG Fangping ; WANG Minxiao ; SUN Song; LI Chaolun; ZHANG Yongshan
发表期刊	Advances in Polar Science
ISSN	1674-9928
	2013-06-15
出版年	2013
卷号	v.24;No.51 期号:02 页码:44-52
文献类型	CNKI期刊论文
摘要	Polar zooplankton are particularly sensitive to climate change, and have been used as rapid-responders to indicate climate-induced changes in the fragile Antarctic ecosystem. DNA barcoding provides an alternative approach for rapid zooplankton species identification. Ninety-four specimens belonging to 32 Antarctic zooplankton species were barcoded to construct a comprehensive reference library. An 830 to 1 050 base-pair region of the mitochondrial cytochrome c oxidase subunit I (mtCOI) gene was obtained as DNA barcodes. The intraspecific variation of the gene ranged from 0 to 2.6% (p-distance), with an average of 0.67% (SD=0.67%). The distance between species within the same genera ranged from 0.1% (Calanus) to 29.3%, with an average of 15.3% (SD=8.4%). The morphological and genetic similarities between Calanus propinquus and C. simillimus raise new questions about the taxonomic status of C. simillimus. With the exception of the two Calanus species, the intraspecific genetic divergence was much smaller than the interspecific divergence among congeneric species, confirming the existence of a barcode gap for Antarctic zooplankton. In addition, species other than Calanus sp. formed a monophyletic group. Therefore, we have confirmed DNA barcoding as an accurate and efficient approach for zooplankton identification in the Antarctic area (except for Hydromedusa, Tunicata, and other gelatinous zooplankton). Indicator vector analysis further confirmed this conclusion. The new primer sets issued here may facilitate the study of Antarctic marine zooplankton species composition by environmental metagenetic analysis.
关键词	Southern Ocean, DNA barcode, Calanus, zooplankton, high-throughput analysis
CNKI专辑号	A;
CNKI专辑名称	基础科学;
CNKI专题号	A006;
CNKI专题名称	生物学;
分类号	Q958.8
语种	英文;
资助项目(基金）	supported by the Chinese Polar Environment Comprehensive Investigation & Assessment Programmes (Grant no.CHINARE2013-01-05) ; the Institute of Oceanology,Chinese Academy of Sciences (IOCAS) Funding (Grant no.2012IO060102)
文献类型	CNKI期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/190853
专题	中国科学院海洋研究所
作者单位	1.KeyLaboratoryofMarineEcologyandEnvironmentalSciences,InstituteofOceanology,ChineseAcademyofSciences 2.UniversityofChineseAcademyofSciences
推荐引用方式 GB/T 7714	CHENG Fangping,WANG Minxiao,SUN Song,et al. DNA barcoding of Antarctic marine zooplankton for species identification and recognition. 2013.