Genome Editing in the Olive Flounder (Paralichthys olivaceus) Using CRISPR/Cas9 and a Simple Microinjection System

IOCAS-IR

	Genome Editing in the Olive Flounder (Paralichthys olivaceus) Using CRISPR/Cas9 and a Simple Microinjection System
	TAN Xungang; WANG Ling; WU Zhihao ; JIAO Shuang ; WANG Lijuan; ZOU Yuxia ; JIANG Jingteng ; YOU Feng
发表期刊	Journal of Ocean University of China
ISSN	1672-5182
	2021-12-06
出版年	2021
卷号	v.20 期号:06 页码:237-245
文献类型	CNKI期刊论文
摘要	The whole-genome sequence of the olive flounder(Paralichthys olivaceus) provides a basis for gene functional analyses,which is important for the aquaculture industry. Understanding gene function will help us to select better economic traits such as fast growth and better culture conditions, which further will increase the aquaculture output. Gene knockout is an important reverse genetics approach for in vivo studies of gene function. In this study, the CRISPR/Cas9 genome editing method with a microinjection system using a simple braked needle was employed in olive flounder. After injection in embryos, green fluorescent protein expression was detected in 40% of larvae. The proportion of normal-hatched larvae was approximately 50%. Different mutations, including short indels and fragment deletions, were found in our test genes gsdf and myomaker. Additionally, we detected more than one mutation in a single larva. In summary, our microinjection technique and CRISPR/Cas9 can be applied to study gene functions in olive flounder.
关键词	CRISPR/Cas9 gsdf microinjection myomaker needle with brake olive flounder
CNKI专辑号	A;D;
CNKI专辑名称	基础科学;农业科技;
CNKI专题号	D052;
CNKI专题名称	水产和渔业;
分类号	S917.4
收录类别	中科院核心 ; 中科院扩展
语种	英文;
资助项目(基金）	supported by the National Natural Science Foundation of China (Nos. 31672636, 31772834, and 31972774) ; the National Key R&D Program of China (Nos. 2018YFD0901202 and 2018YFD0900202) ; the Key Research and Development Program of Shandong Province, China (No. 2019GHY1120070)
文献类型	CNKI期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/187571
专题	中国科学院海洋研究所
作者单位	1.CASandShandongProvinceKeyLaboratoryofExperimentalMarineBiology,CenterforOceanMega-Science,InstituteofOceanology,ChineseAcademyofSciences 2.LaboratoryforMarineBiologyandBiotechnology,QingdaoNationalLaboratoryforMarineScienceandTechnology 3.UniversityofChineseAcademyofSciences 4.WeihaiFisheryTechnologyPromotionStation 5.ShenghangAquaticScienceandTechnologyCo.,Ltd.
推荐引用方式 GB/T 7714	TAN Xungang,WANG Ling,WU Zhihao,et al. Genome Editing in the Olive Flounder (Paralichthys olivaceus) Using CRISPR/Cas9 and a Simple Microinjection System. 2021.