Study on the Characteristics and Mechanism of Inorganic Nitrogen Nutrients Preferential Assimilation by <i>Phaeocystis globosa</i>

doi:10.3390/jmse12060989

	Study on the Characteristics and Mechanism of Inorganic Nitrogen Nutrients Preferential Assimilation by Phaeocystis globosa
	Guo, Honghong 1,2,3; Wang, Wentao2,3,4 ; Han, Jishu 1; Zhu, Jianan2,3 ; Wang, Zhongshi2,3,4 ; He, Liyan 2,3; Song, Xiuxian2,3,4 ; Yu, Zhiming2,3,4
	2024-06-01
发表期刊	JOURNAL OF MARINE SCIENCE AND ENGINEERING
卷号	12 期号:6 页码:15
通讯作者	Wang, Wentao([email protected])
摘要	Phaeocystis globosa is an important harmful algal species that is globally distributed. Previous studies have indicated that P. globosa preferentially uptake nitrate, but the underlying mechanism is still unclear. To further verify this preference and reveal the underlying mechanism, the assimilation rates of nitrate and ammonium by P. globosa at different concentrations was quantitatively studied by using a nitrogen stable isotope (N-15) tracer technique, and the regulatory mechanism was determined from the physiological and biochemical responses. The findings revealed that the preferential assimilation of nitrate by P. globosa was influenced by the ambient ammonium concentration. When the ambient concentration of ammonium was less than approximately 3.5 mu mol center dot L-1, the assimilation rates of nitrate form P. globosa were as high as 1.05 x 10(-5) mu mol center dot L-1 center dot h(-1)center dot cell(-1). Even though the nitrate assimilation in P. globosa was inhibited at ammonium concentrations greater than this threshold, nitrate assimilation could not be completely suppressed. The activity of NR and the expression of related genes in P. globosa were also affected by ammonium. In addition, N-15 signals originally labeled nitrate accumulated in ammonium. This indicated that P. globosa was likely to reduce excess nitrate to ammonium and subsequently release it into the substrate, which might be an important energy dissipation mechanism for P. globosa. These results expand the classical understanding of the utilization of nitrogen nutrients by marine phytoplankton, and offer valuable resources for comprehending the mechanism of harmful algal blooms.
关键词	Phaeocystis globosa nitrate ammonium isotope tracer assimilation
DOI	10.3390/jmse12060989
收录类别	SCI
语种	英语
资助项目	Shandong Provincial Natural Science Foundation of China[ZR2019QD014]; Intergovernmental Innovation Cooperation Project of the Ministry of Science and Technology[2022YFE0136400]; Laoshan Laboratory[LSKJ202203700]; National Natural Science Foundation of China[42306150]
WOS研究方向	Engineering ; Oceanography
WOS类目	Engineering, Marine ; Engineering, Ocean ; Oceanography
WOS记录号	WOS:001257064600001
出版者	MDPI
WOS关键词	NITRATE UPTAKE ; SOUTHERN BIGHT ; NORTH-SEA ; PHYTOPLANKTON ; AMMONIUM ; GROWTH ; INHIBITION ; DYNAMICS ; KINETICS ; RELEASE
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/186444
专题	海洋生态与环境科学重点实验室
通讯作者	Wang, Wentao
作者单位	1.Qingdao Univ Sci & Technol, Coll Chem & Mol Engn, Qingdao 266042, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 3.Qingdao Marine Sci & Technol Ctr, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	海洋生态与环境科学重点实验室
通讯作者单位	海洋生态与环境科学重点实验室
推荐引用方式 GB/T 7714	Guo, Honghong,Wang, Wentao,Han, Jishu,et al. Study on the Characteristics and Mechanism of Inorganic Nitrogen Nutrients Preferential Assimilation by Phaeocystis globosa[J]. JOURNAL OF MARINE SCIENCE AND ENGINEERING,2024,12(6):15.
APA	Guo, Honghong.,Wang, Wentao.,Han, Jishu.,Zhu, Jianan.,Wang, Zhongshi.,...&Yu, Zhiming.(2024).Study on the Characteristics and Mechanism of Inorganic Nitrogen Nutrients Preferential Assimilation by Phaeocystis globosa.JOURNAL OF MARINE SCIENCE AND ENGINEERING,12(6),15.
MLA	Guo, Honghong,et al."Study on the Characteristics and Mechanism of Inorganic Nitrogen Nutrients Preferential Assimilation by Phaeocystis globosa".JOURNAL OF MARINE SCIENCE AND ENGINEERING 12.6(2024):15.