Characterization of 12 single nucleotide polymorphisms (SNPs) in Pacific abalone, Haliotis discus hannai | |
Qi, Hai-Gang1,2; Liu, Xiao1; Zhang, Guo-Fan1; Liu, X, Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China | |
2008-09-01 | |
发表期刊 | MOLECULAR ECOLOGY RESOURCES
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ISSN | 1755-098X |
卷号 | 8期号:5页码:974-976 |
文章类型 | Article |
摘要 | We report here for the first time 12 polymorphic single nucleotide polymorphisms (SNPs) in a commercially important gastropod, Pacific abalone (Haliotis discus hannai) that were identified by searching expressed sequence tag database. These SNP loci (seven nuclear and five mitochondrial SNPs) were polymorphic among 37 wild abalone individuals, based on a four-primer allele-specific polymerase chain reaction analysis. All loci had two alleles and the minor allele frequency ranged from 0.027 to 0.473. For the seven nuclear SNPs, the expected and observed heterozygosities ranged from 0.053 to 0.499 and from 0.054 to 0.811, respectively.; We report here for the first time 12 polymorphic single nucleotide polymorphisms (SNPs) in a commercially important gastropod, Pacific abalone (Haliotis discus hannai) that were identified by searching expressed sequence tag database. These SNP loci (seven nuclear and five mitochondrial SNPs) were polymorphic among 37 wild abalone individuals, based on a four-primer allele-specific polymerase chain reaction analysis. All loci had two alleles and the minor allele frequency ranged from 0.027 to 0.473. For the seven nuclear SNPs, the expected and observed heterozygosities ranged from 0.053 to 0.499 and from 0.054 to 0.811, respectively. |
关键词 | As-pcr Haliotis Discus Hannai Pacific Abalone Polymorphism Snp |
学科领域 | Biochemistry & Molecular Biology ; Ecology ; Evolutionary Biology |
DOI | 10.1111/j.1755-0998.2008.02118.x |
URL | 查看原文 |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000258997100008 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qdio.ac.cn/handle/337002/1862 |
专题 | 海洋生物技术研发中心 |
通讯作者 | Liu, X, Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
推荐引用方式 GB/T 7714 | Qi, Hai-Gang,Liu, Xiao,Zhang, Guo-Fan,et al. Characterization of 12 single nucleotide polymorphisms (SNPs) in Pacific abalone, Haliotis discus hannai[J]. MOLECULAR ECOLOGY RESOURCES,2008,8(5):974-976. |
APA | Qi, Hai-Gang,Liu, Xiao,Zhang, Guo-Fan,&Liu, X, Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China.(2008).Characterization of 12 single nucleotide polymorphisms (SNPs) in Pacific abalone, Haliotis discus hannai.MOLECULAR ECOLOGY RESOURCES,8(5),974-976. |
MLA | Qi, Hai-Gang,et al."Characterization of 12 single nucleotide polymorphisms (SNPs) in Pacific abalone, Haliotis discus hannai".MOLECULAR ECOLOGY RESOURCES 8.5(2008):974-976. |
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