Green and eco-friendly method for the extraction of chitin from crab shells (Chionoecetes opilio) with Lactiplantibacillus plantarum and Pseudomonas aeruginosa

doi:10.1007/s13399-024-05970-y

IOCAS-IR > 实验海洋生物学重点实验室

	Green and eco-friendly method for the extraction of chitin from crab shells (Chionoecetes opilio) with Lactiplantibacillus plantarum and Pseudomonas aeruginosa
	Zhou, Yi1,3 ; Yang, Haoyue1 ; Li, Guantian 1; Xing, Ronge1,2 ; Liu, Song 1,2; Li, Kecheng 1,2; Li, Rongfeng 1,2; Yu, Huahua1,2
	2024-07-31
发表期刊	BIOMASS CONVERSION AND BIOREFINERY
ISSN	2190-6815
页码	16
通讯作者	Xing, Ronge([email protected])
摘要	There are few reports on the extraction of chitin from crab shells using microbiological methods. In this work, the strain Lactiplantibacillus plantarum (LA01) for demineralization (DM) and the strain Pseudomonas aeruginosa (PS01) for deproteinization (DP) were screened from probiotic powders, soil, seawater, mud of aquaculture farm, seamount, and marine cold spring. After optimizing the fermentation conditions, PS01 followed by LA01 was chosen as the better inoculation sequence for two-step fermentation process to extract chitin from crab shells (Chionoecetes opilio). The chitin obtained by fermentation was analyzed by FTIR, XRD, SEM, and TGA. Results showed that 97.55 +/- 0.15% of calcium carbonate and 73.49 +/- 0.25% of protein were eliminated. The strong connection between protein and chitin in the crab shells was speculated as the reason of protein residues. A similar structure was observed in the crab chitin according to the results of structural analysis compared with commercial chitin, indicating the efficacy of microbiological methods used for chitin extraction from crab shells. The presence of porous and fibrous structure was observed in crab chitin according to the images of SEM. The organic acids in the supernatant of LA01 were mainly lactic acid (26,190.31 mg/L) and acetic acid (799.85 mg/L). The results of antioxidant activity assays showed that the fermentation supernatant possessed strong antioxidant activity, in which the scavenging activity of DPPH free radicals (96.50 +/- 1.68%) in LA01 was higher than that of 1.5 g/L ascorbic acid solution (96.18 +/- 0.43%), with 0.24 +/- 0.00 mg/mL of total phenolic and 7.54 +/- 0.31 mg/mL of exopolysaccharide.
关键词	Chitin extraction Crab shells Microbiological method Antioxidant activity
DOI	10.1007/s13399-024-05970-y
收录类别	SCI
语种	英语
资助项目	The Key R&D Program (Major Scientific and Technological Innovation Project) of Shandong Province, China
WOS研究方向	Energy & Fuels ; Engineering
WOS类目	Energy & Fuels ; Engineering, Chemical
WOS记录号	WOS:001285090800004
出版者	SPRINGER HEIDELBERG
WOS关键词	MOLECULAR-WEIGHT CHITIN ; CHITOSAN ; SHRIMP ; WASTE ; FERMENTATION ; PRODUCTS
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/186199
专题	实验海洋生物学重点实验室
通讯作者	Xing, Ronge
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Marine Sci & Technol Ctr, Lab Marine Drugs & Bioprod, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100000, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Zhou, Yi,Yang, Haoyue,Li, Guantian,et al. Green and eco-friendly method for the extraction of chitin from crab shells (Chionoecetes opilio) with Lactiplantibacillus plantarum and Pseudomonas aeruginosa[J]. BIOMASS CONVERSION AND BIOREFINERY,2024:16.
APA	Zhou, Yi.,Yang, Haoyue.,Li, Guantian.,Xing, Ronge.,Liu, Song.,...&Yu, Huahua.(2024).Green and eco-friendly method for the extraction of chitin from crab shells (Chionoecetes opilio) with Lactiplantibacillus plantarum and Pseudomonas aeruginosa.BIOMASS CONVERSION AND BIOREFINERY,16.
MLA	Zhou, Yi,et al."Green and eco-friendly method for the extraction of chitin from crab shells (Chionoecetes opilio) with Lactiplantibacillus plantarum and Pseudomonas aeruginosa".BIOMASS CONVERSION AND BIOREFINERY (2024):16.