Mitochondrial alternative oxidase pathway accelerates non-motile cell germination by enhancing respiratory carbon metabolism and maintaining redox poise in Haematococcus pluvialis

doi:10.1016/j.biortech.2024.130729

IOCAS-IR > 实验海洋生物学重点实验室

	Mitochondrial alternative oxidase pathway accelerates non-motile cell germination by enhancing respiratory carbon metabolism and maintaining redox poise in Haematococcus pluvialis
	Li, Jing 1,2,4; Zhang, Litao 1,2,3; Yu, Wenjie1,2,3 ; Zhang, Mengjie1,2,3 ; Chen, Feng 1,2,4; Liu, Jianguo 1,2,3,5
	2024-06-01
发表期刊	BIORESOURCE TECHNOLOGY
ISSN	0960-8524
卷号	402 页码:10
通讯作者	Liu, Jianguo([email protected])
摘要	Low efficiency of the cultivation process is a major obstacle in the commercial production of Haematococcus pluvialis . Germination of red, non -motile cells is an efficient strategy for rapid acquisition of zoospores. However, the regulatory mechanisms associated with germination remain unexplored. In the present study, it was confirmed that the mitochondrial alternative oxidase (AOX) pathway accelerates H. pluvialis cell germination, and the regulatory mechanisms were clarified. When the AOX pathway was inhibited, the transcriptomic and metabonomic data revealed a downregulation in respiratory carbon metabolism and nucleotide synthesis due to NADH accumulation. This observation suggested that AOX promoted the rapid consumption of NADH, which accelerated carbohydrate and lipid catabolism, thereby producing carbon skeletons for DNA replication through respiratory metabolism. Moreover, AOX could potentially enhance germination by disturbing the abscisic acid signaling pathway. These findings provide novel insights for developing industrial cultivation models based on red -cell -germination for achieving rapid proliferation of H. pluvialis .
关键词	Rapid proliferation Lipid catabolism Reducing equivalents Abscisic acid
DOI	10.1016/j.biortech.2024.130729
收录类别	SCI
语种	英语
资助项目	Strategic Priority Research Program of the Chinese Academy of Sciences, China[XDB42000000]; TS Industrial Experts Program[LJNY202102]; Na- tional Natural Science Foundation of China, China[32370409]; Na- tional Natural Science Foundation of China, China[32302979]; Science & Technology Specific Projects in Agri- cultural High-tech Industrial Demonstration Area of the Yellow River Delta[2022SZX13]
WOS研究方向	Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS类目	Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels
WOS记录号	WOS:001238115300001
出版者	ELSEVIER SCI LTD
WOS关键词	CHLAMYDOMONAS-REINHARDTII ; INVOLVEMENT ; EXPRESSION ; STRESS ; GENE ; WALL ; CO2
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/186027
专题	实验海洋生物学重点实验室
通讯作者	Liu, Jianguo
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Shandong Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Natl Ctr Technol Innovat Comprehens Utilizat Salin, Academician Workstat Agr High Tech Ind Area Yellow, Dongying 257300, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Li, Jing,Zhang, Litao,Yu, Wenjie,et al. Mitochondrial alternative oxidase pathway accelerates non-motile cell germination by enhancing respiratory carbon metabolism and maintaining redox poise in Haematococcus pluvialis[J]. BIORESOURCE TECHNOLOGY,2024,402:10.
APA	Li, Jing,Zhang, Litao,Yu, Wenjie,Zhang, Mengjie,Chen, Feng,&Liu, Jianguo.(2024).Mitochondrial alternative oxidase pathway accelerates non-motile cell germination by enhancing respiratory carbon metabolism and maintaining redox poise in Haematococcus pluvialis.BIORESOURCE TECHNOLOGY,402,10.
MLA	Li, Jing,et al."Mitochondrial alternative oxidase pathway accelerates non-motile cell germination by enhancing respiratory carbon metabolism and maintaining redox poise in Haematococcus pluvialis".BIORESOURCE TECHNOLOGY 402(2024):10.