In vivo immunological activity of chitosan-derived nanoparticles

doi:10.1016/j.ijbiomac.2024.130105

IOCAS-IR > 实验海洋生物学重点实验室

	In vivo immunological activity of chitosan-derived nanoparticles
	Xu, Chaojie; Xing, Ronge1 ; Liu, Song ; Qin, Yukun; Li, Kecheng ; Yu, Huahua; Li, Pengcheng
	2024-03-01
发表期刊	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN	0141-8130
卷号	262 页码:9
通讯作者	Xing, Ronge([email protected])
摘要	Chitosan has been studied as an immunomodulator, but few studies have used chitosan derivatives as adjuvants alone. After a preliminary study, we found that nanoparticles prepared from chitosan derivatives had better cellular immune activity when used as an adjuvant. Therefore, animal experiments were conducted to further investigate the performance and mechanism of these nanoparticles as immune adjuvants. We injected mice with the chitosan nanoparticle vaccine and measured the expression levels of immunoglobulins, immune factors, and immune genes in tissues and tissue sections. The results showed that C236-HACC-OVA (C2,3,6-chitosan sulfatechitosan quaternary ammonium salt-ovalbumin) and NO-HACC-OVA (NO-carboxymethyl chitosan-chitosan quaternary ammonium salt-ovalbumin) nanoparticles can significantly improve the secretion of the immune factors IL -6, TNF, and IL-1 beta. The level of IgG1 was highly significant after administering both nanoparticles, but IgG2 was not significant in mice. Three immune factors (IL -4, IL -6, and IL -17) were secreted at high levels in mouse serum at a nanoparticle dose of 0.3 mg/mouse. These nanoparticles also have high safety in the liver, kidney, and spleen of mice. This study proves the possibility of using chitosan derivative nanoparticles as vaccine adjuvants. These data further indicate that chitosan derivative nanoparticles have potential for use as vaccine adjuvants and demonstrate that polysaccharides have a unique position in green vaccine research.
关键词	Chitosan-derived Nanoparticles Immunity Adjuvant Vaccine
DOI	10.1016/j.ijbiomac.2024.130105
收录类别	SCI
语种	英语
资助项目	Qingdao Marine Science and Technology Center[2022QNLM030003-2]; The Key R&D Program (Major Scientific and Technological Innovation Project) of Shandong Province, China[2022CXGC020413]; The Key R&D Program (Major Scientific and Technological Innovation Project) of Shandong Province, China[2023CXGC010412]; Shandong Provincial Natural Science Foundation[ZR2023QD180]; China Postdoctoral Science Foundation project[2023M733530]; Natural Science Foundation of Shandong Province[ZR2022YQ37]; Qingdao Postdoctoral Project; Key R&D Program (Major Scientific and Technological Innovation Project) of Shandong Province, China[2023CXGC010412]
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science
WOS记录号	WOS:001187950400001
出版者	ELSEVIER
WOS关键词	IMMUNE-RESPONSES ; CARBOXYMETHYL CHITOSAN ; VACCINE ; ADJUVANT ; DELIVERY ; CHITIN
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/184954
专题	实验海洋生物学重点实验室
通讯作者	Xing, Ronge
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Drugs & Bioprod, 1 Wenhai Rd, Qingdao 266237, Peoples R China
推荐引用方式 GB/T 7714	Xu, Chaojie,Xing, Ronge,Liu, Song,et al. In vivo immunological activity of chitosan-derived nanoparticles[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2024,262:9.
APA	Xu, Chaojie.,Xing, Ronge.,Liu, Song.,Qin, Yukun.,Li, Kecheng.,...&Li, Pengcheng.(2024).In vivo immunological activity of chitosan-derived nanoparticles.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,262,9.
MLA	Xu, Chaojie,et al."In vivo immunological activity of chitosan-derived nanoparticles".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 262(2024):9.