Comparative transcriptome analyses reveal changes of gene expression in turbot (Scophthalmus maximus) embryos exposed to different LED light spectra and potential photosensitive function of non-visual opsins

doi:10.1016/j.aqrep.2024.101948

IOCAS-IR > 实验海洋生物学重点实验室

	Comparative transcriptome analyses reveal changes of gene expression in turbot (Scophthalmus maximus) embryos exposed to different LED light spectra and potential photosensitive function of non-visual opsins
	Wu, Lele1 ; Li, Jun 2; Liu, Feng 2; Song, Zongcheng 3; Song, Changbin 4; Xu, Shihong 2; Yue, Xinlu 3; Li, Xian 1,5
	2024-04-01
发表期刊	AQUACULTURE REPORTS
ISSN	2352-5134
卷号	35 页码:13
通讯作者	Li, Xian([email protected])
摘要	This study describes the transcriptome profiles of turbot (Scophthalmus maximus) embryos exposed to various light spectra, helping to better understand the importance of light spectrum on the development of fish embryo. When exposed to red, orange, green or blue light, up-regulated genes were significantly enriched into lipid, carbohydrate metabolism, indicating enhanced energy consumption compared with full-spectrum light. Molecular functions related to signal transduction, such as supramolecular polymers/complexes/fibers, protein dimerization, were down-regulated in red, orange, and blue groups, while calcium ion binding was up-regulated under green light, indicating that turbot embryo might be more sensitive to green light. The transcription regulator activity and DNA binding transcription factor activity related to retina and nervous system development were down-regulated in orange, green and blue groups, suggesting full light spectrum could be more conducive to the development of nervous system. Moreover, orange light could be advantageous for enhancing the antioxidant capacity, while green light caused abnormal expression of some development related genes. The non-visual photoreceptors with vertebrate ancient opsin and exorhodopsin expression in hypothalamus, hindbrain and pineal area played mainly photosensitive roles. This study provides new insights into the molecular mechanisms underlying photosensitive-phototransduction-physiological-development process in fish embryos and further confirmed the feasibility of utilizing orange light and full spectrum light to establish optimized developmental light spectrum environment in turbot embryo incubation systems.
关键词	Light spectrum Phototransduction Scophthalmus maximus Embryo Transcriptome
DOI	10.1016/j.aqrep.2024.101948
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[41976122]; National Natural Science Foundation of China[32273104]; Young Talent Program of Ocean University of China[862201013156]; China Agriculture Research System[CARS-47]
WOS研究方向	Fisheries
WOS类目	Fisheries
WOS记录号	WOS:001172104100001
出版者	ELSEVIER
WOS关键词	SOLE SOLEA-SENEGALENSIS ; PINEAL ORGAN ; PHOTOPERIOD ; INTENSITY ; ZEBRAFISH ; ONTOGENY ; PIGMENT ; SYSTEM ; RETINA ; GROWTH
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/184790
专题	实验海洋生物学重点实验室
通讯作者	Li, Xian
作者单位	1.Ocean Univ China, Fisheries Coll, Key Lab Mariculture, Minist Educ, Qingdao 266001, Peoples R China 2.Inst Oceanol, Chinese Acad Sci, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Weihai Shenghang Aquat Prod Sci & Technol Co Ltd, Weihai 264200, Peoples R China 4.Inst Semicond, Chinese Acad Sci, Beijing 100083, Peoples R China 5.Ocean Univ China, Fisheries Coll, Qingdao 266001, Peoples R China
推荐引用方式 GB/T 7714	Wu, Lele,Li, Jun,Liu, Feng,et al. Comparative transcriptome analyses reveal changes of gene expression in turbot (Scophthalmus maximus) embryos exposed to different LED light spectra and potential photosensitive function of non-visual opsins[J]. AQUACULTURE REPORTS,2024,35:13.
APA	Wu, Lele.,Li, Jun.,Liu, Feng.,Song, Zongcheng.,Song, Changbin.,...&Li, Xian.(2024).Comparative transcriptome analyses reveal changes of gene expression in turbot (Scophthalmus maximus) embryos exposed to different LED light spectra and potential photosensitive function of non-visual opsins.AQUACULTURE REPORTS,35,13.
MLA	Wu, Lele,et al."Comparative transcriptome analyses reveal changes of gene expression in turbot (Scophthalmus maximus) embryos exposed to different LED light spectra and potential photosensitive function of non-visual opsins".AQUACULTURE REPORTS 35(2024):13.