The Responses of Alternative Splicing during Heat Stress in the Pacific White Shrimp Litopenaeus vannamei

doi:10.3390/genes14071473

IOCAS-IR > 实验海洋生物学重点实验室

	The Responses of Alternative Splicing during Heat Stress in the Pacific White Shrimp Litopenaeus vannamei
	Zhang, Xiaoxi1,2,3,4 ; Zhang, Xiaojun 1,2,3,4; Yuan, Jianbo 1,2,3,4; Li, Fuhua 1,2,3,4
	2023-07-01
发表期刊	GENES
卷号	14 期号:7 页码:12
通讯作者	Li, Fuhua([email protected])
摘要	Heat tolerance is increasingly becoming a crucial trait for aquaculture species in the face of rapidly changing climate conditions. Alternative splicing (AS) is a vital mechanism within cells that modulates gene abundance and functional diversity, enabling organisms to effectively respond to diverse stressful conditions, including thermal stress. However, it is still uncertain whether AS contributes to heat tolerance in shrimp. In this study, we conducted an extensive transcriptome analysis on the Pacific white shrimp, Litopenaeus vannamei, revealing a total of 1267, 987, and 130 differential AS events (DAS) in the gill, hepatopancreas, and muscle, respectively, following exposure to heat stress. Among all of the DAS events, exon skipping (ES) was the predominant form of splicing modification observed. Interestingly, a minor portion of DAS genes exhibited overlap across the three tissues, implying that heat stress exerts unique effects on various tissue types. Moreover, the functional enrichment analysis demonstrated that commonly identified DAS genes were primarily associated with the "spliceosome" pathway, indicating that the AS of splicing-related genes played a crucial role in the response to heat stress. Our findings also revealed that heat stress tended to induce longer mRNA isoforms through differential alternative 3 & PRIME; splice site (A3SS) events. Notably, A3SS events exhibited the highest proportion of maintained open reading frames (ORFs) under heat stress. Interestingly, we observed a limited overlap between the genes exhibiting DAS and those showing differential gene expression (DEG), indicating that AS may function as a distinct regulatory mechanism independent of transcriptional regulation in response to heat stress. This is the first comprehensive study on AS in crustacea species under heat stress, which broadens our understanding of the regulatory mechanisms governing the crustaceans' response to environmental stress, providing valuable insights for the aquaculture breeding of shrimp and other aquatic animals.
关键词	alternative splicing heat stress Litopenaeus vannamei RNA-Seq
DOI	10.3390/genes14071473
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[42206091]; National Natural Science Foundation of China[31972782]; National Natural Science Foundation of China[42176105]; China Postdoctoral Science Foundation[2021M703249]; Agriculture Research System-48 of China[CARS-48]; Taishan Scholars Program
WOS研究方向	Genetics & Heredity
WOS类目	Genetics & Heredity
WOS记录号	WOS:001038500600001
出版者	MDPI
WOS关键词	GENOME-WIDE ANALYSIS ; TRANSCRIPTOME ; ADAPTATION ; MECHANISMS ; EXPRESSION ; PROTEINS
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/182596
专题	实验海洋生物学重点实验室
通讯作者	Li, Fuhua
作者单位	1.Chinese Acad Sci, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Key Lab Breeding Biotechnol & Sustainable Aquacult, Wuhan 430072, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Zhang, Xiaoxi,Zhang, Xiaojun,Yuan, Jianbo,et al. The Responses of Alternative Splicing during Heat Stress in the Pacific White Shrimp Litopenaeus vannamei[J]. GENES,2023,14(7):12.
APA	Zhang, Xiaoxi,Zhang, Xiaojun,Yuan, Jianbo,&Li, Fuhua.(2023).The Responses of Alternative Splicing during Heat Stress in the Pacific White Shrimp Litopenaeus vannamei.GENES,14(7),12.
MLA	Zhang, Xiaoxi,et al."The Responses of Alternative Splicing during Heat Stress in the Pacific White Shrimp Litopenaeus vannamei".GENES 14.7(2023):12.