Oyster studies reveal the duplication and functional diversification of Bivalvia caspase-8 genes

doi:10.1007/s00343-022-2129-7

IOCAS-IR > 实验海洋生物学重点实验室

	Oyster studies reveal the duplication and functional diversification of Bivalvia caspase-8 genes
	Deng, Shaoxi1,2 ; Qu, Tao 1,4; Zhang, Guofan 1,2,3; Xu, Fei 1,2,3
	2023-06-22
发表期刊	JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN	2096-5508
页码	13
通讯作者	Xu, Fei([email protected])
摘要	Caspase-8, first classified as a pro-apoptotic caspase, is considered to have arisen from duplication with caspase-10 and involves multiple immune and inflammatory responses in mammals. However, few are known on the phylogeny and function of caspase-8 in molluscs, one of the largest phyla in marine invertebrates. In this study, we conducted phylogenetic and functional analysis on molluscan caspase-8-like genes. Results indicate that duplication occurred in molluscan caspase-8-like genes, resulting in at least two caspase-8 copies in some groups of bivalves. Additional studies in Pacific oyster Crassostrea gigas showed different spatio-temporal expression patterns and subcellular localizations of CgCaspase-8-1 and CgCaspase-8-2. While no interaction was observed between CgCaspase-8-2 and CgFADD, the adaptor molecule in apoptosis, yeast two-hybrid and co-immunoprecipitation assays suggested the interaction between CgCaspase-8-1 and CgFADD, indicating its pro-apoptotic function. In addition, CgCaspase-8-1 showed interaction with the CARD domain of CgRIG-I. Together with two NF-& kappa;B subunits (Cgp105 and CgRel), their transcripts were up-regulated in response to poly(I:C) stimuli, supporting the immune function of both pro, and anti-inflammation. The results provide insight into the evolution and functional diversification of Bivalvia caspase-8 genes.
关键词	caspase-8 apoptosis metamorphosis inflammation Crassostrea gigas
DOI	10.1007/s00343-022-2129-7
收录类别	SCI
语种	英语
WOS研究方向	Marine & Freshwater Biology ; Oceanography
WOS类目	Limnology ; Oceanography
WOS记录号	WOS:001011988000001
出版者	SCIENCE PRESS
WOS关键词	NF-KAPPA-B ; TNF RECEPTOR ; CELL-DEATH ; APOPTOSIS ; ACTIVATION ; PATHWAY ; CLONING ; DOMAIN ; RECOGNITION ; ILLUSTRATOR
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/182359
专题	实验海洋生物学重点实验室
通讯作者	Xu, Fei
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266000, Peoples R China 4.Gansu Prov Hosp, NHC Key Lab Diag & Therapy Gastrointestinal Tumor, Lanzhou 730000, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Deng, Shaoxi,Qu, Tao,Zhang, Guofan,et al. Oyster studies reveal the duplication and functional diversification of Bivalvia caspase-8 genes[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2023:13.
APA	Deng, Shaoxi,Qu, Tao,Zhang, Guofan,&Xu, Fei.(2023).Oyster studies reveal the duplication and functional diversification of Bivalvia caspase-8 genes.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,13.
MLA	Deng, Shaoxi,et al."Oyster studies reveal the duplication and functional diversification of Bivalvia caspase-8 genes".JOURNAL OF OCEANOLOGY AND LIMNOLOGY (2023):13.