Comparative Transcriptome Analysis Reveals the Adaptation Mechanism to High Salinity in Litopenaeus vannamei

doi:10.3389/fmars.2022.864338

IOCAS-IR > 实验海洋生物学重点实验室

	Comparative Transcriptome Analysis Reveals the Adaptation Mechanism to High Salinity in Litopenaeus vannamei
	Luo, Zheng1,2 ; Yu, Yang1,3 ; Zhang, Qian1 ; Bao, Zhenning 1,2; Xiang, Jianhai1 ; Li, Fuhua 1,3,4,5
	2022-06-02
发表期刊	FRONTIERS IN MARINE SCIENCE
卷号	9 页码:14
通讯作者	Yu, Yang([email protected]) ; Li, Fuhua([email protected])
摘要	As an euryhaline commercial species, the Pacific white shrimp Litopenaeus vannamei can be cultured under a wide range of salinities. However, different families showed various tolerance to high salinity. In order to elucidate the underlying mechanisms, a comparative transcriptome approach was used to find the differences between tolerant families and susceptible families at the transcriptional level. There were 98 and 58 differentially expressed genes (DEGs) between tolerant and susceptible families under normal and high-salinity conditions, respectively. The DEGs upregulated in tolerant families were mostly enriched in the biological process of response to a stimulus. Nine crustacyanin (CRCN)-encoding genes in this Gene Ontology (GO) term showed significantly higher expression levels in the tolerant families than those in susceptible families, suggesting that crustacyanins might play important roles in shrimp tolerance to high salinity. After high-salinity treatment, a total of 233 and 109 DEGs were identified from tolerant and susceptible families, respectively. Serine-type endopeptidase activity, serine-type peptidase activity, and serine hydrolase activity were the top three GO terms in the tolerant families after high-salinity treatment. Further analysis illustrated that these three GO terms were also the top three enriched GO terms for the DEGs specifically upregulated in the tolerant families after high-salinity stress. Meanwhile, four genes annotated in serine proteases families in these three GO terms were upregulated in the tolerant families but not changed in the susceptible families after high-salinity stress. These results indicated that serine proteases should play key roles in shrimp tolerance to high salinity. This study provides important information for understanding the mechanism of shrimp tolerance to high-salinity stress and would be useful for further molecular breeding of shrimp varieties with high-salinity tolerance.
关键词	high-salinity comparative transcriptome tolerant families susceptible families Litopenaeus vannamei
DOI	10.3389/fmars.2022.864338
收录类别	SCI
语种	英语
资助项目	National Key R&D Program of China[2018YFD0900303]; Shandong Provincial Natural Science Foundation[ZR2020MC191]; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA24030105]; China Agriculture Research System of MOF and MARA
WOS研究方向	Environmental Sciences & Ecology ; Marine & Freshwater Biology
WOS类目	Environmental Sciences ; Marine & Freshwater Biology
WOS记录号	WOS:000812101200001
出版者	FRONTIERS MEDIA SA
引用统计	被引频次：10[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/179513
专题	实验海洋生物学重点实验室
通讯作者	Yu, Yang; Li, Fuhua
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China 5.Chinese Acad Sci, Innovat Seed Design, Wuhan, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Luo, Zheng,Yu, Yang,Zhang, Qian,et al. Comparative Transcriptome Analysis Reveals the Adaptation Mechanism to High Salinity in Litopenaeus vannamei[J]. FRONTIERS IN MARINE SCIENCE,2022,9:14.
APA	Luo, Zheng,Yu, Yang,Zhang, Qian,Bao, Zhenning,Xiang, Jianhai,&Li, Fuhua.(2022).Comparative Transcriptome Analysis Reveals the Adaptation Mechanism to High Salinity in Litopenaeus vannamei.FRONTIERS IN MARINE SCIENCE,9,14.
MLA	Luo, Zheng,et al."Comparative Transcriptome Analysis Reveals the Adaptation Mechanism to High Salinity in Litopenaeus vannamei".FRONTIERS IN MARINE SCIENCE 9(2022):14.

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