Gonad Transcriptome and Whole-Genome DNA Methylation Analyses Reveal Potential Sex Determination/Differentiation Mechanisms of the Deep-Sea Mussel Gigantidas platifrons

doi:10.3389/fmars.2022.856291

	Gonad Transcriptome and Whole-Genome DNA Methylation Analyses Reveal Potential Sex Determination/Differentiation Mechanisms of the Deep-Sea Mussel Gigantidas platifrons
	Zhong, Zhaoshan1,2,3 ; Wang, Minxiao 1,2,3; Chen, Hao1,2,3 ; Wang, Hao 1,2,3; Zhang, Huan 1,2,3; Zhou, Li 1,2,3; Sun, Yan 1,2,3; Cao, Lei1,2,3 ; Lian, Chao 1,2,3; Li, Mengna 1,2,3,4; Li, Chaolun1,2,3,4
	2022-04-28
发表期刊	FRONTIERS IN MARINE SCIENCE
卷号	9 页码:15
通讯作者	Li, Chaolun([email protected])
摘要	Gigantidas platifrons is one of the most dominant deep-sea mussels in cold seeps ecosystems in the South China sea. Studies have shown that deep-sea mussels are a gonochoristic species, however, little is known about the molecular mechanisms of sex determination in G. platifrons. In this study, RNA-seq and WGBS methylation analysis were performed on adult G. platifrons gonads to identify potential sex-related genes and generate a comprehensive analysis of sex determination in deep-sea mussels. A total of 5923 genes were identified as differentially expressed between the ovaries and testes, of which 2711 were female-biased and 3212 were male-biased. Among them, 161 genes may participate in the sex determination, and we found that DMRT2 may play an important role in male sex determination, and FOXL2, Wnt7, and beta-catenin may have impact on female sex determination. Moreover, common expression patterns were found in majority of the sex-related genes such as FOXL2, beta-catenin, and genes in SOX family, suggesting the sex determination mechanisms of mussels in different habitats were conserved. The 5mC levels of transcription start sites (TSS2K) were significantly higher in sex-related genes than other DEGs in both gonads. Positive correlation was observed between sex-related genes expression and methylation in male, however, the effects of the DNA methylation on gene expression were complex in female. In short, we argue that sex determination mechanisms of deep-sea mussel G. platifrons is mainly controlled by genetic, and the methylation may have a regulation role on male sex determination or differentiation.
关键词	deep-sea mussel Gigantidas platifrons gonad transcriptome methylation sex determination and differentiation
DOI	10.3389/fmars.2022.856291
收录类别	SCI
语种	英语
WOS研究方向	Environmental Sciences & Ecology ; Marine & Freshwater Biology
WOS类目	Environmental Sciences ; Marine & Freshwater Biology
WOS记录号	WOS:000794553600001
出版者	FRONTIERS MEDIA SA
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/179225
专题	海洋生态与环境科学重点实验室
通讯作者	Li, Chaolun
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Ctr Deep Sea Res, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Zhong, Zhaoshan,Wang, Minxiao,Chen, Hao,et al. Gonad Transcriptome and Whole-Genome DNA Methylation Analyses Reveal Potential Sex Determination/Differentiation Mechanisms of the Deep-Sea Mussel Gigantidas platifrons[J]. FRONTIERS IN MARINE SCIENCE,2022,9:15.
APA	Zhong, Zhaoshan.,Wang, Minxiao.,Chen, Hao.,Wang, Hao.,Zhang, Huan.,...&Li, Chaolun.(2022).Gonad Transcriptome and Whole-Genome DNA Methylation Analyses Reveal Potential Sex Determination/Differentiation Mechanisms of the Deep-Sea Mussel Gigantidas platifrons.FRONTIERS IN MARINE SCIENCE,9,15.
MLA	Zhong, Zhaoshan,et al."Gonad Transcriptome and Whole-Genome DNA Methylation Analyses Reveal Potential Sex Determination/Differentiation Mechanisms of the Deep-Sea Mussel Gigantidas platifrons".FRONTIERS IN MARINE SCIENCE 9(2022):15.

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