Genome Editing in the Olive Flounder (Paralichthys olivaceus) Using CRISPR/Cas9 and a Simple Microinjection System

doi:10.1007/s11802-021-4772-6

IOCAS-IR > 实验海洋生物学重点实验室

	Genome Editing in the Olive Flounder (Paralichthys olivaceus) Using CRISPR/Cas9 and a Simple Microinjection System
	Tan Xungang1,2 ; Wang Ling1,2,3 ; Wu Zhihao 1,2; Jiao Shuang 1,2; Wang Lijuan1,2 ; Zou Yuxia 1,2; Jiang Jingteng 4,5; You Feng 1,2
	2021-12-01
发表期刊	JOURNAL OF OCEAN UNIVERSITY OF CHINA
ISSN	1672-5182
卷号	20 期号:6 页码:1528-1536
通讯作者	Tan Xungang([email protected]) ; You Feng([email protected])
摘要	The whole-genome sequence of the olive flounder (Paralichthys olivaceus) provides a basis for gene functional analyses, which is important for the aquaculture industry. Understanding gene function will help us to select better economic traits such as fast growth and better culture conditions, which further will increase the aquaculture output. Gene knockout is an important reverse genetics approach for in vivo studies of gene function. In this study, the CRISPR/Cas9 genome editing method with a microinjection system using a simple braked needle was employed in olive flounder. After injection in embryos, green fluorescent protein expression was detected in 40% of larvae. The proportion of normal-hatched larvae was approximately 50%. Different mutations, including short indels and fragment deletions, were found in our test genes gsdf and myomaker. Additionally, we detected more than one mutation in a single larva. In summary, our microinjection technique and CRISPR/Cas9 can be applied to study gene functions in olive flounder.
关键词	CRISPR Cas9 gsdf microinjection myomaker needle with brake olive flounder
DOI	10.1007/s11802-021-4772-6
收录类别	SCI
语种	英语
WOS研究方向	Oceanography
WOS类目	Oceanography
WOS记录号	WOS:000720813700021
出版者	OCEAN UNIV CHINA
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/177213
专题	实验海洋生物学重点实验室
通讯作者	Tan Xungang; You Feng
作者单位	1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab ForMarine Biol & Biotechnol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Weihai Fishery Technol Promot Stn, Weihai 264200, Peoples R China 5.Shenghang Aquat Sci & Technol Co Ltd, Weihai 264200, Peoples R China
第一作者单位	中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Tan Xungang,Wang Ling,Wu Zhihao,et al. Genome Editing in the Olive Flounder (Paralichthys olivaceus) Using CRISPR/Cas9 and a Simple Microinjection System[J]. JOURNAL OF OCEAN UNIVERSITY OF CHINA,2021,20(6):1528-1536.
APA	Tan Xungang.,Wang Ling.,Wu Zhihao.,Jiao Shuang.,Wang Lijuan.,...&You Feng.(2021).Genome Editing in the Olive Flounder (Paralichthys olivaceus) Using CRISPR/Cas9 and a Simple Microinjection System.JOURNAL OF OCEAN UNIVERSITY OF CHINA,20(6),1528-1536.
MLA	Tan Xungang,et al."Genome Editing in the Olive Flounder (Paralichthys olivaceus) Using CRISPR/Cas9 and a Simple Microinjection System".JOURNAL OF OCEAN UNIVERSITY OF CHINA 20.6(2021):1528-1536.

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