Morpho-molecular description of a new HAB species, Pseudocochlodinium profundisulcus gen. et sp. nov., and its LSU rRNA gene based genetic diversity and geographical distribution

doi:10.1016/j.hal.2021.102098

	Morpho-molecular description of a new HAB species, Pseudocochlodinium profundisulcus gen. et sp. nov., and its LSU rRNA gene based genetic diversity and geographical distribution
	Hu, Zhangxi1,2,3 ; Xu, Ning 4; Gu, Haifeng 5; Chai, Zhaoyang 1; Takahashi, Kazuya 6; Li, Zhun 7; Deng, Yunyan 1,2,3; Iwataki, Mitsunori 6; Matsuoka, Kazumi 8; Tang, Ying Zhong 1,2,3
	2021-08-01
发表期刊	HARMFUL ALGAE
ISSN	1568-9883
卷号	108 页码:15
通讯作者	Iwataki, Mitsunori([email protected]) ; Matsuoka, Kazumi([email protected]) ; Tang, Ying Zhong([email protected])
摘要	Harmful algal blooms (HABs) caused by an unknown dinoflagellate species have frequently occurred in the Pearl River Estuary, China Since 2006. These blooms were associated with severe water discoloration and economic losses, ranging from several km(2) to 300 km(2) with the maximum recorded cell density being 2.77 x 10(7) cells.L-1. This unknown dinoflagellate species was initially identified as Cochlodinium geminatum and subsequently reclassified as Polykrikos geminatus. However, after reviewing the original descriptions for Cochlodinium geminatum sensu Schutt (1895) and the genus Polykrikos, we considered this species is incongruent with their original descriptions. Further morphological examinations and particularly phylogenetic analyses based on the SSU and partial LSU rRNA genes of isolates and resting cysts from China and Japan prompted us to consider it a new species of a new genus. This new species was proposed to be Pseudocochlodinium profundisulcus gen. et sp. nov., based on its open comma-shaped apical structure complex (ASC), cingulum encircling the cell less than one and a half turns, a deep sulcus with a torsion of a half turn, either single cell or cell chain consisting of two cells with the same number of nuclei and zooids, the resting cyst bearing lobed ornaments, and the evolutionary distances from Polykrikos (and others) on the phylogenetic trees constructed using the concatenated SSU and partial LSU rRNA gene sequences. Metabarcoding investigation of surface sediment samples collected in China revealed that the species to be widely present along the entire Chinese coast with the highest abundance in the South China Sea. Further re-analysis of the Tara Oceans metabarcoding dataset targeting the SSU rRNA gene V9 domain suggested a global distribution of this new genus. Phylogenetic analyses on 46 OTUs (average length: similar to 552 bases) of its LSU rRNA gene sequences (mainly D1-D2 domains) obtained from surface sediment samples revealed intraspecific genetic diversity of this species. Interestingly, based on the different distributions and the abundance of these OTUs along the coast of China, this species appeared to have expanded its distribution from the South China Sea to the northern Yellow Sea, or preferred a warm water habitat. We consider that the present work improves the taxonomy and provides important insights into the biogeography of Pseudocochlodinium profundisulcus.
关键词	Apical structure complex Cochlodinium geminatum Geographical distribution LSU rRNA gene Pseudocochlodinium profundisulcus Resting cyst SSU rRNA gene
DOI	10.1016/j.hal.2021.102098
收录类别	SCI
语种	英语
资助项目	Science & Technology Basic Resources Investigation Program of China[2018FY100200]; National Natural Science Founda-tion of China[41976134]; National Natural Science Founda-tion of China[41576159]; National Natural Science Founda-tion of China[41776125]
WOS研究方向	Marine & Freshwater Biology
WOS类目	Marine & Freshwater Biology
WOS记录号	WOS:000698761100001
出版者	ELSEVIER
引用统计	被引频次：13[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/176431
专题	海洋生态与环境科学重点实验室
通讯作者	Iwataki, Mitsunori; Matsuoka, Kazumi; Tang, Ying Zhong
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Lab Marine Ecol & Environm Sci, Qingdao Natl Lab Marine Sci & Technol, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China 4.Jinan Univ, Inst Hydrobiol, Guangzhou 510632, Peoples R China 5.Minist Nat Resources, Inst Oceanog 3, Xiamen 361005, Peoples R China 6.Univ Tokyo, Grad Sch Agr & Life Sci, Tokyo 1138657, Japan 7.Korea Res Inst Biosci & Biotechnol, Biol Resource Ctr, Korean Collect Type Cultures KCTC, Jeongeup 56212, South Korea 8.Nagasaki Univ, Inst East China Sea Res, Nagasaki 8512213, Japan
第一作者单位	海洋生态与环境科学重点实验室; 中国科学院海洋大科学研究中心
通讯作者单位	海洋生态与环境科学重点实验室; 中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Hu, Zhangxi,Xu, Ning,Gu, Haifeng,et al. Morpho-molecular description of a new HAB species, Pseudocochlodinium profundisulcus gen. et sp. nov., and its LSU rRNA gene based genetic diversity and geographical distribution[J]. HARMFUL ALGAE,2021,108:15.
APA	Hu, Zhangxi.,Xu, Ning.,Gu, Haifeng.,Chai, Zhaoyang.,Takahashi, Kazuya.,...&Tang, Ying Zhong.(2021).Morpho-molecular description of a new HAB species, Pseudocochlodinium profundisulcus gen. et sp. nov., and its LSU rRNA gene based genetic diversity and geographical distribution.HARMFUL ALGAE,108,15.
MLA	Hu, Zhangxi,et al."Morpho-molecular description of a new HAB species, Pseudocochlodinium profundisulcus gen. et sp. nov., and its LSU rRNA gene based genetic diversity and geographical distribution".HARMFUL ALGAE 108(2021):15.

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