A Toll-like receptor identified in Gigantidas platifrons and its potential role in the immune recognition of endosymbiotic methane oxidation bacteria

doi:10.7717/peerj.11282

	A Toll-like receptor identified in Gigantidas platifrons and its potential role in the immune recognition of endosymbiotic methane oxidation bacteria
	Li, Mengna 1,2,3,4; Chen, Hao1,2,3,5,6 ; Wang, Minxiao 1,2,3,5,6; Zhong, Zhaoshan1,2,3,5,6 ; Wang, Hao 1,2,3,5,6; Zhou, Li 1,2,3,5,6; Zhang, Huan 1,2,3,5,6; Li, Chaolun1,2,3,4,5,6
	2021-04-30
发表期刊	PEERJ
ISSN	2167-8359
卷号	9 页码:21
通讯作者	Li, Chaolun([email protected])
摘要	Symbiosis with chemosynthetic bacteria is an important ecological strategy for the deep-sea megafaunas including mollusks, tubeworms and crustacean to obtain nutrients in hydrothermal vents and cold seeps. How the megafaunas recognize symbionts and establish the symbiosis has attracted much attention. Bathymodiolinae mussels are endemic species in both hydrothermal vents and cold seeps while the immune recognition mechanism underlying the symbiosis is not well understood due to the nonculturable symbionts. In previous study, a lipopolysaccharide (LPS) pull-down assay was conducted in Gigantidas platifrons to screen the pattern recognition receptors potentially involved in the recognition of symbiotic methane-oxidizing bacteria (MOB). Consequently, a total of 208 proteins including GpTLR13 were identified. Here the molecular structure, expression pattern and immune function of GpTLR13 were further analyzed. It was found that GpTLR13 could bind intensively with the lipid A structure of LPS through surface plasmon resonance analysis. The expression alternations of GpTLR13 transcripts during a 28-day of symbiont-depletion assay were investigated by real-time qPCR. As a result, a robust decrease of GpTLR13 transcripts was observed accompanying with the loss of symbionts, implying its participation in symbiosis. In addition, GpTLR13 transcripts were found expressed exclusively in the bacteriocytes of gills of G. platifrons by in situ hybridization. It was therefore speculated that GpTLR13 may be involved in the immune recognition of symbiotic methane-oxidizing bacteria by specifically recognizing the lipid A structure of LPS. However, the interaction between GpTLR13 and symbiotic MOB was failed to be addressed due to the nonculturable symbionts. Nevertheless, the present result has provided with a promising candidate as well as a new approach for the identification of symbiont-related genes in Bathymodiolinae mussels.
关键词	Gigantidas platifrons Methane-oxidizing bacteria Symbiosis Immune recognition Toll like receptor Pattern recognition receptors
DOI	10.7717/peerj.11282
收录类别	SCI
语种	英语
资助项目	Strategic Priority Research Program of the Chinese Academy of Sciences[XDA22050303]; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB42020401]; Key Research Program of Frontier Sciences, CAS[ZDBS-LY-DQC032]; National Key Research and Development Program of China[2018YFC0310800]; National Natural Science Foundation of China[42030407]; National Natural Science Foundation of China[42076091]; National Natural Science Foundation of China[41906103]; Key Deployment Project of Centre for Ocean Mega-Research of Science, CAS[COMS2020Q02]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000647021700004
出版者	PEERJ INC
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/170917
专题	海洋生态与环境科学重点实验室深海极端环境与生命过程研究中心
通讯作者	Li, Chaolun
作者单位	1.Chinese Acad Sci, Ctr Deep Sea Res, Qingdao, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci CODR, Qingdao, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, KLMEES, Qingdao, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China 5.Qingdao Natl Lab Marine Sci & Technol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 6.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所; 中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Li, Mengna,Chen, Hao,Wang, Minxiao,et al. A Toll-like receptor identified in Gigantidas platifrons and its potential role in the immune recognition of endosymbiotic methane oxidation bacteria[J]. PEERJ,2021,9:21.
APA	Li, Mengna.,Chen, Hao.,Wang, Minxiao.,Zhong, Zhaoshan.,Wang, Hao.,...&Li, Chaolun.(2021).A Toll-like receptor identified in Gigantidas platifrons and its potential role in the immune recognition of endosymbiotic methane oxidation bacteria.PEERJ,9,21.
MLA	Li, Mengna,et al."A Toll-like receptor identified in Gigantidas platifrons and its potential role in the immune recognition of endosymbiotic methane oxidation bacteria".PEERJ 9(2021):21.

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