Edwardsiella tarda outer membrane protein 1 is a virulence factor and a protective immunogen

doi:10.1016/j.aquaculture.2021.736596

IOCAS-IR > 实验海洋生物学重点实验室

	Edwardsiella tarda outer membrane protein 1 is a virulence factor and a protective immunogen
	Jin, Cheng-dong1,2,3 ; Li, Mo-fei1,2
	2021-06-30
发表期刊	AQUACULTURE
ISSN	0044-8486
卷号	539 页码:9
通讯作者	Li, Mo-fei([email protected])
摘要	The bacterial outer membrane proteins are important components of bacterial cell. Edwardsiella tarda is a vital bacterial pathogen to a wide range of aquaculture animals. In this study, we examined the function of an outer membrane protein of E. tarda (named Omp1Et). Omp1Et was found to localize on the surface of E. tarda, and recombinant Omp1Et (rOmp1Et) was able to interact with flounder peripheral blood leukocytes. Compared to the wild type, the omp1Et knockout TX01?omp1 was significantly reduced in motility, biofilm formation, attachment and invasion into host cells, dissemination in host tissues, and inducing host mortality. Furthermore, TX01?omp1 was significantly impaired in the ability to survive under low pH and oxidizing conditions. When introduced into flounder as a subunit vaccine, rOmp1Et induced strong immune protection against E. tarda challenge. Taken together, these results indicate that Omp1Et is a surface-located virulence factor of E. tarda and rOmp1Et can induce effective immune-protection as a submit vaccine.
关键词	Edwardsiella tarda Outer membrane protein Virulence Vaccine
DOI	10.1016/j.aquaculture.2021.736596
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[31972831]; National Key Research and Development Program of China[2018YFD0900501]; Youth Innovation Promotion Association of Chinese Academy of Sciences[2017249]; Huiquan Young Scholar Program of Institute of Oceanology, CAS; Taishan Scholar Program of Shandong Province
WOS研究方向	Fisheries ; Marine & Freshwater Biology
WOS类目	Fisheries ; Marine & Freshwater Biology
WOS记录号	WOS:000644225600001
出版者	ELSEVIER
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/170869
专题	实验海洋生物学重点实验室
通讯作者	Li, Mo-fei
作者单位	1.Chinese Acad Sci, CAS Ctr Ocean Megasci, Inst Oceanol, CAS Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, 1 Wenhai Rd, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Jin, Cheng-dong,Li, Mo-fei. Edwardsiella tarda outer membrane protein 1 is a virulence factor and a protective immunogen[J]. AQUACULTURE,2021,539:9.
APA	Jin, Cheng-dong,&Li, Mo-fei.(2021).Edwardsiella tarda outer membrane protein 1 is a virulence factor and a protective immunogen.AQUACULTURE,539,9.
MLA	Jin, Cheng-dong,et al."Edwardsiella tarda outer membrane protein 1 is a virulence factor and a protective immunogen".AQUACULTURE 539(2021):9.

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