Preparation and Neutralization Efficacy of Novel Jellyfish Antivenoms against Cyanea nozakii Toxins

doi:10.3390/toxins13020165

IOCAS-IR > 实验海洋生物学重点实验室

	Preparation and Neutralization Efficacy of Novel Jellyfish Antivenoms against Cyanea nozakii Toxins
	Li, Rongfeng 1,2; Yu, Huahua1,2 ; Li, Aoyu1 ; Yu, Chunlin1,3 ; Li, Pengcheng1,2
	2021-02-01
发表期刊	TOXINS
卷号	13 期号:2 页码:16
通讯作者	Li, Rongfeng([email protected]) ; Li, Pengcheng([email protected])
摘要	Jellyfish stings are a common issue globally, particularly in coastal areas in the summer. Victims can suffer pain, itching, swelling, shock, and even death. Usually, hot water, vinegar, or alumen is used to treat the normal symptoms of a jellyfish sting. However, a specific antivenom may be an effective treatment to deal with severe jellyfish stings. Cyanea nozakii often reach a diameter of 60 cm and are responsible for hundreds of thousands of stings per year in coastal Chinese waters. However, there has been no specific C. nozakii antivenom until now, and so the development of this antivenom is very important. Herein, we collected C. nozakii antisera from tentacle extract venom immunized rabbits and purified the immunoglobulin (IgG) fraction antivenom (AntiCnTXs). Subsequently, two complete procedures to produce a refined F(ab')(2) type of antivenom (F(ab')(2)-AntiCnTXs) and Fab type of antivenom (Fab-AntiCnTXs) by multiple optimizations and purification were established. The neutralization efficacy of these three types of antivenoms was compared and analyzed in vitro and in vivo, and the results showed that all types of antibodies displayed some neutralization effect on the lethality of C. nozakii venom toxins, with the neutralization efficacy as follows: F(ab')(2)-AntiCnTXs >= AntiCnTXs > Fab-AntiCnTXs. This study describes the preparation of novel C. nozakii jellyfish antivenom preparations towards the goal of developing a new, effective treatment for jellyfish stings.
关键词	jellyfish Cyanea nozakii antivenom AntiCnTXs F(ab’ )(2)-AntiCnTXs Fab-AntiCnTXs
DOI	10.3390/toxins13020165
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[41876164]; National Natural Science Foundation of China[41776163]; Natural Science Foundation of Shandong Province[ZR2019QD012]; National Key Research and Development Program of China[2017YFE0111100-04]
WOS研究方向	Food Science & Technology ; Toxicology
WOS类目	Food Science & Technology ; Toxicology
WOS记录号	WOS:000623280000001
出版者	MDPI
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/169592
专题	实验海洋生物学重点实验室
通讯作者	Li, Rongfeng; Li, Pengcheng
作者单位	1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Drugs & Bioprod, 1 Wenhai Rd, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Coll Earth & Planetary Sci, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Li, Rongfeng,Yu, Huahua,Li, Aoyu,et al. Preparation and Neutralization Efficacy of Novel Jellyfish Antivenoms against Cyanea nozakii Toxins[J]. TOXINS,2021,13(2):16.
APA	Li, Rongfeng,Yu, Huahua,Li, Aoyu,Yu, Chunlin,&Li, Pengcheng.(2021).Preparation and Neutralization Efficacy of Novel Jellyfish Antivenoms against Cyanea nozakii Toxins.TOXINS,13(2),16.
MLA	Li, Rongfeng,et al."Preparation and Neutralization Efficacy of Novel Jellyfish Antivenoms against Cyanea nozakii Toxins".TOXINS 13.2(2021):16.

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