Effects of chronic nitrate exposure on the intestinal morphology, immune status, barrier function, and microbiota of juvenile turbot (Scophthalmus maximus)

doi:10.1016/j.ecoenv.2020.111287

IOCAS-IR > 实验海洋生物学重点实验室

	Effects of chronic nitrate exposure on the intestinal morphology, immune status, barrier function, and microbiota of juvenile turbot (Scophthalmus maximus)
	Yu, Jiachen 1,2,3,4; Wang, Yanfeng 1,2,3; Xiao, Yongshuang 1,2,3; Li, Xian 1,2,3; Xu, Xiaojie1,2,3,4 ; Zhao, Haixia1,2,3,4 ; Wu, Lele1,2,3,4 ; Li, Jun 1,2,3
	2021
发表期刊	ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
ISSN	0147-6513
卷号	207 页码:11
通讯作者	Li, Jun([email protected])
摘要	Coming along with high water reuse in sustainable and intensive recirculating aquaculture systems (RASs), the waste products of fish in rearing water is continuously accumulated. Nitrate, the final product of biological nitrification processes, which may cause aquatic toxicity to fish in different degrees when exposed for a long time. Therefore, the present study was conducted to evaluate the impact of chronic nitrate exposure on intestinal morphology, immune status, barrier function, and microbiota of juvenile turbot. For that, groups of juvenile turbot were exposed to 0 (control check, CK), 50 (low nitrate, L), 200 (medium nitrate, M), and 400 (high nitrate, H) mg L-1 nitrate-N in small-sized recirculating aquaculture systems. After the 60-day experiment period, we found that exposure to a high concentration of nitrate-N caused obvious pathological damages to the intestine; for instance, atrophy of intestinal microvilli and necrosis in the lamina propria. Quantitative real-time PCR analysis revealed a significant downregulation of the barrier forming tight junction genes like occludin, claudin-like etc. under H treatment (P < 0.05). Intestinal MUC-2 expression also decreased significantly in the nitrate treatment groups compared to that in the control (P < 0.05). Additionally, the expression of HSP70 and HSP90 heat-shock proteins, toll-like receptor-3 (TLR-3), interleukin-1 beta (IL-1 beta), and tumor necrosis factor-alpha (TNF-alpha) significantly increased (P < 0.05), whereas that of transforming growth factor-beta (TGF-beta), lysozyme (LYS), and insulin-like growth factor-I (IGF-I) significantly decreased with H treatment (P < 0.05). The results also revealed that intestinal microbial community was changed following nitrate exposure and could alter the alpha-diversity and beta-diversity. Specifically, the proportion of intrinsic flora decreased, whereas that of the potential pathogens significantly increased with M and H treatments (P < 0.05). In conclusion, chronic nitrate exposure could weaken the barrier function and disturb the composition of intestinal microbiota in marine teleosts, thereby harming their health condition.
关键词	Scophthalmus maximus Nitrate exposure Intestinal health condition Intestinal microbiota
DOI	10.1016/j.ecoenv.2020.111287
收录类别	SCI
语种	英语
资助项目	National Key Research and Development Program[2018YFD0901204]; China Agriculture Research System[CARS-47]; STS project[KFZD-SW-106]; STS project[ZSSD-019]; STS project[2017T3017]; STS project[KFJ-STS-QYZX-020]; Qingdao National Laboratory for Marine Science and Technology[2018SDKJ0502-2]; Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02]; National Natural Science Foundation of China[31672672]
WOS研究方向	Environmental Sciences & Ecology ; Toxicology
WOS类目	Environmental Sciences ; Toxicology
WOS记录号	WOS:000592453200008
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
引用统计	被引频次：23[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/169310
专题	实验海洋生物学重点实验室
通讯作者	Li, Jun
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Ctr Ocean MegaSci, 7 Nanhai Rd, Qingdao 266071, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China
第一作者单位	中国科学院海洋研究所; 中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋研究所; 中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Yu, Jiachen,Wang, Yanfeng,Xiao, Yongshuang,et al. Effects of chronic nitrate exposure on the intestinal morphology, immune status, barrier function, and microbiota of juvenile turbot (Scophthalmus maximus)[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2021,207:11.
APA	Yu, Jiachen.,Wang, Yanfeng.,Xiao, Yongshuang.,Li, Xian.,Xu, Xiaojie.,...&Li, Jun.(2021).Effects of chronic nitrate exposure on the intestinal morphology, immune status, barrier function, and microbiota of juvenile turbot (Scophthalmus maximus).ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,207,11.
MLA	Yu, Jiachen,et al."Effects of chronic nitrate exposure on the intestinal morphology, immune status, barrier function, and microbiota of juvenile turbot (Scophthalmus maximus)".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 207(2021):11.

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