CRISPR/Cas9-mediated mutation reveals Pax6 is essential for development of the compound eye in Decapoda Exopalaemon carinicauda

doi:10.1016/j.ydbio.2020.07.001

IOCAS-IR > 实验海洋生物学重点实验室

	CRISPR/Cas9-mediated mutation reveals Pax6 is essential for development of the compound eye in Decapoda Exopalaemon carinicauda
	Gao, Yi 1,2,3; Zhang, Xiaoxi1,4 ; Zhang, Xiaojun 1,2,3; Yuan, Jianbo 1,2,3; Xiang, Jianhai1,2 ; Li, Fuhua 1,2,3
	2020-09-15
发表期刊	DEVELOPMENTAL BIOLOGY
ISSN	0012-1606
卷号	465 期号:2 页码:157-167
通讯作者	Li, Fuhua([email protected])
摘要	The compound eye in crustaceans is a main eye type in the animal kingdom, knowledge about the mechanism to determine the development of compound eye is very limited. Paired box protein 6 (Pax6) is generally regarded as a master regulator for eye development. In the present study, a genome-based analysis of the Pax6 gene in the ridge tail white prawn Exopalaemon carinicauda was performed and two members of Pax6 homologs, named Ec-Eyeless (EcEy) and Ec-Twin of eyeless (EcToy) were identified. To understand the function of these two homologs of Pax6 gene in the prawn, the CRISPR/Cas9 genome editing technique was applied to generate EcEy and EcToy knock-out (KO) prawns and their phenotypes were analyzed. The surviving EcEy-KO embryos and larvae exhibited severe abnormal eye morphology, suggesting that EcEy is necessary for the compound eye development in prawn, while no mutant phenotype was found in EcToy-KO individuals. These findings highlighted the conservative role of Pax6 gene in the compound eye formation, and the functional differentiation between EcEy and EcToy gene may reveal a novel regulating mechanism of Pax6 on the compound eye development in the decapods. These data will provide important information for understanding the regulation mechanism for crustacean compound eye development.
关键词	Development Compound eye Pax6 CRISPR/Cas9 Crustaceans
DOI	10.1016/j.ydbio.2020.07.001
收录类别	SCI
语种	英语
资助项目	National Key R&D Program of China[2018YFD0901301]; National Key R&D Program of China[2018YFD0900404]; Major Science and Technology Innovation Projects in Shandong Province[2018SDKJ0302-5]; National Natural Science Foundation of China[31702320]
WOS研究方向	Developmental Biology
WOS类目	Developmental Biology
WOS记录号	WOS:000567412300008
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
引用统计	被引频次：17[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/168735
专题	实验海洋生物学重点实验室
通讯作者	Li, Fuhua
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Gao, Yi,Zhang, Xiaoxi,Zhang, Xiaojun,et al. CRISPR/Cas9-mediated mutation reveals Pax6 is essential for development of the compound eye in Decapoda Exopalaemon carinicauda[J]. DEVELOPMENTAL BIOLOGY,2020,465(2):157-167.
APA	Gao, Yi,Zhang, Xiaoxi,Zhang, Xiaojun,Yuan, Jianbo,Xiang, Jianhai,&Li, Fuhua.(2020).CRISPR/Cas9-mediated mutation reveals Pax6 is essential for development of the compound eye in Decapoda Exopalaemon carinicauda.DEVELOPMENTAL BIOLOGY,465(2),157-167.
MLA	Gao, Yi,et al."CRISPR/Cas9-mediated mutation reveals Pax6 is essential for development of the compound eye in Decapoda Exopalaemon carinicauda".DEVELOPMENTAL BIOLOGY 465.2(2020):157-167.

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