Cloning and Comparative Studies of Seaweed Trehalose-6-Phosphate Synthase Genes

doi:10.3390/md8072065

IOCAS-IR > 实验海洋生物学重点实验室

	Cloning and Comparative Studies of Seaweed Trehalose-6-Phosphate Synthase Genes
	Wang, Guoliang 2,3; Zhao, Ge 2; Feng, Yanbin 2; Xuan, Jinsong 2; Sun, Jianwei 2; Guo, Baotai 4; Jiang, Guoyong 4; Weng, Manli 2; Yao, Jianting 1; Wang, Bin2 ; Duan, Delin 1; Liu, Tao 3; Duan, DL, Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
	2010-07-01
发表期刊	MARINE DRUGS
ISSN	1660-3397
卷号	8 期号:7 页码:2065-2079
文章类型	Article
摘要	The full-length cDNA sequence (3219 base pairs) of the trehalose-6-phosphate synthase gene of Porphyra yezoensis (PyTPS) was isolated by RACE-PCR and deposited in GenBank (NCBI) with the accession number AY729671. PyTPS encodes a protein of 908 amino acids before a stop codon, and has a calculated molecular mass of 101,591 Daltons. The PyTPS protein consists of a TPS domain in the N-terminus and a putative TPP domain at the C-terminus. Homology alignment for PyTPS and the TPS proteins from bacteria, yeast and higher plants indicated that the most closely related sequences to PyTPS were those from higher plants (OsTPS and AtTPS5), whereas the most distant sequence to PyTPS was from bacteria (EcOtsAB). Based on the identified sequence of the PyTPS gene, PCR primers were designed and used to amplify the TPS genes from nine other seaweed species. Sequences of the nine obtained TPS genes were deposited in GenBank (NCBI). All 10 TPS genes encoded peptides of 908 amino acids and the sequences were highly conserved both in nucleotide composition (>94%) and in amino acid composition (>96%). Unlike the TPS genes from some other plants, there was no intron in any of the 10 isolated seaweed TPS genes.; The full-length cDNA sequence (3219 base pairs) of the trehalose-6-phosphate synthase gene of Porphyra yezoensis (PyTPS) was isolated by RACE-PCR and deposited in GenBank (NCBI) with the accession number AY729671. PyTPS encodes a protein of 908 amino acids before a stop codon, and has a calculated molecular mass of 101,591 Daltons. The PyTPS protein consists of a TPS domain in the N-terminus and a putative TPP domain at the C-terminus. Homology alignment for PyTPS and the TPS proteins from bacteria, yeast and higher plants indicated that the most closely related sequences to PyTPS were those from higher plants (OsTPS and AtTPS5), whereas the most distant sequence to PyTPS was from bacteria (EcOtsAB). Based on the identified sequence of the PyTPS gene, PCR primers were designed and used to amplify the TPS genes from nine other seaweed species. Sequences of the nine obtained TPS genes were deposited in GenBank (NCBI). All 10 TPS genes encoded peptides of 908 amino acids and the sequences were highly conserved both in nucleotide composition (>94%) and in amino acid composition (>96%). Unlike the TPS genes from some other plants, there was no intron in any of the 10 isolated seaweed TPS genes.
关键词	Comparative Genomics Of Tps Genes Gene Cloning Race-pcr Seaweed Trehalose-6-phosphate Synthase Gene
学科领域	Chemistry, Medicinal
DOI	10.3390/md8072065
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000280347100007
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/1681
专题	实验海洋生物学重点实验室
通讯作者	Duan, DL, Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 3.Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China 4.Qingdao Agr Univ, Coll Life Sci, Qingdao 266109, Peoples R China
推荐引用方式 GB/T 7714	Wang, Guoliang,Zhao, Ge,Feng, Yanbin,et al. Cloning and Comparative Studies of Seaweed Trehalose-6-Phosphate Synthase Genes[J]. MARINE DRUGS,2010,8(7):2065-2079.
APA	Wang, Guoliang.,Zhao, Ge.,Feng, Yanbin.,Xuan, Jinsong.,Sun, Jianwei.,...&Duan, DL, Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China.(2010).Cloning and Comparative Studies of Seaweed Trehalose-6-Phosphate Synthase Genes.MARINE DRUGS,8(7),2065-2079.
MLA	Wang, Guoliang,et al."Cloning and Comparative Studies of Seaweed Trehalose-6-Phosphate Synthase Genes".MARINE DRUGS 8.7(2010):2065-2079.

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