Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Integrated proteomics and metabolomics analysis reveals the antifungal mechanism of the C-coordinated O-carboxymethyl chitosan Cu (II) complex | |
Ma, Yuzhen1,2,3; Yu, Huahua1,2 ; Liu, Weixiang1,2; Qin, Yukun1,2; Xing, Ronge1,2; Li, Pengcheng1,2
| |
| 2020-07-15 | |
| 发表期刊 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
 |
| ISSN | 0141-8130 |
| 卷号 | 155页码:1491-1509 |
| 通讯作者 | Yu, Huahua([email protected]) ; Li, Pengcheng([email protected]) |
| 摘要 | With wide application in agriculture, copper fungicides have undergone three stages of development: inorganic copper, synthetic organic copper, and natural organic copper. Using chitin/chitosan (CS) as a substrate, the natural organic copper fungicide C-coordinated O-carboxymethyl chitosan Cu(II) complex (O-CSLn-Cu) was developed in the laboratory. Taking Phytophthora capsici Leonian as an example, we explored the antifungal mechanism of O-CSLn-Cu by combining tandem mass tag (TMT)-based proteomics with non-targeted liquid chromatography-mass spectrometry (LC-MS)-based metabolomics. A total of 1172 differentially expressed proteins were identified by proteomics analysis. According to the metabolomics analysis, 93 differentially metabolites were identified. Acetyl-CoA-related and membrane localized proteins showed significant differences in the proteomics analysis. Most of the differential expressed metabolites were distributed in the cytoplasm, followed by mitochondria. The integrated analysis revealed that O-CSLn-Cu could induce the "Warburg effect", with increased glycolysis in the cytoplasm and decreased metabolism in the mitochondria. Therefore, P. capsici Leonian had to compensate for ATP loss in the TCA cycle by increasing the glycolysis rate. However, this metabolic shift could not prevent the death of P. capsici Leonian. To verify this hypothesis, a series of biological experiments, such as scanning electron microscopy (SEM), transmission electron microscopy (TEM), and enzyme activity measurements were carried out. The results suggest that O-CSLn-Cu causes mitochondrial injury, which consequently leads to excessive ROS levels and insufficient ATP levels, thereby killing P. capsici Leonian. (C) 2019 Elsevier B.V. All rights reserved. |
| 关键词 | Natural organic-based copper O-CSLn-Cu Phytophthora capsici Leonian Proteomics-metabonomics integrated analysis Antifungal mechanism |
| DOI | 10.1016/j.ijbiomac.2019.11.127 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | theNational Key Research and Development Program of China[2018YFC0311305]; Key Research and Development Program of Shandong Province[2018GHY115017]; Key Research and Development Program of Shandong Province[2018GHY115008]; Key Research and Development Program of Shandong Province[2019GHY112015] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Polymer Science |
| WOS类目 | Biochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science |
| WOS记录号 | WOS:000536122500155 |
| 出版者 | ELSEVIER |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/167606 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Yu, Huahua; Li, Pengcheng |
| 作者单位 | 1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Drugs & Bioprod, 1 Wenhai Rd, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 第一作者单位 | 中国科学院海洋大科学研究中心 |
| 通讯作者单位 | 中国科学院海洋大科学研究中心 |
| 推荐引用方式 GB/T 7714 | Ma, Yuzhen,Yu, Huahua,Liu, Weixiang,et al. Integrated proteomics and metabolomics analysis reveals the antifungal mechanism of the C-coordinated O-carboxymethyl chitosan Cu (II) complex[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2020,155:1491-1509. |
| APA | Ma, Yuzhen,Yu, Huahua,Liu, Weixiang,Qin, Yukun,Xing, Ronge,&Li, Pengcheng.(2020).Integrated proteomics and metabolomics analysis reveals the antifungal mechanism of the C-coordinated O-carboxymethyl chitosan Cu (II) complex.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,155,1491-1509. |
| MLA | Ma, Yuzhen,et al."Integrated proteomics and metabolomics analysis reveals the antifungal mechanism of the C-coordinated O-carboxymethyl chitosan Cu (II) complex".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 155(2020):1491-1509. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Integrated proteomic（13837KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论