| Preparation, characterization and antioxidant activity of two partially N-acetylated chitotrioses | |
Li, Kecheng1,2; Liu, Song1; Xing, Ronge1 ; Qin, Yukun1; Li, Pengcheng1; Xing, RG
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| 2013-02-15 | |
| 发表期刊 | CARBOHYDRATE POLYMERS
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| ISSN | 0144-8617 |
| 卷号 | 92期号:2页码:1730-1736 |
| 文章类型 | Article |
| 摘要 | The preparation of chitooligosaccharides with single degree of polymerization and well-defined degree of acetylation is of significance to identify the components possessing strong biofunction. This study focuses on the preparation of two partially acetylated chitotrioses (N-acetylchitotriose and N,N'-diacetylchitotriose), involving a two-step process: (i) partially N-acetylation of chitotriose and (ii) separation of different acetylated chitotrioses. The chitotriose was N-acetylated and characterized with FT-IR and ESI-MS. N-acetylchitotriose and N,N'-diacetylchitotriose were separated from the acetylated chitotriose mixture by CM Sepharose Fast Flow. Subsequently, the antioxidant activities of two partially acetylated chitotrioses and original chitotriose were investigated, including hydroxyl radical and superoxide radical scavenging activity. The results showed that the activities of the three oligomers were in a dose-dependence manner and related to their degree of acetylation. The N,N'-diacetylchitotriose with high degree of acetylation exhibited the highest antioxidant activity. (C) 2012 Elsevier Ltd. All rights reserved.; The preparation of chitooligosaccharides with single degree of polymerization and well-defined degree of acetylation is of significance to identify the components possessing strong biofunction. This study focuses on the preparation of two partially acetylated chitotrioses (N-acetylchitotriose and N,N'-diacetylchitotriose), involving a two-step process: (i) partially N-acetylation of chitotriose and (ii) separation of different acetylated chitotrioses. The chitotriose was N-acetylated and characterized with FT-IR and ESI-MS. N-acetylchitotriose and N,N'-diacetylchitotriose were separated from the acetylated chitotriose mixture by CM Sepharose Fast Flow. Subsequently, the antioxidant activities of two partially acetylated chitotrioses and original chitotriose were investigated, including hydroxyl radical and superoxide radical scavenging activity. The results showed that the activities of the three oligomers were in a dose-dependence manner and related to their degree of acetylation. The N,N'-diacetylchitotriose with high degree of acetylation exhibited the highest antioxidant activity. (C) 2012 Elsevier Ltd. All rights reserved. |
| 关键词 | Chitotriose N-acetylchitotriose Separation n Antioxidant Activity n '-diacetylchitotriose |
| 学科领域 | Chemistry ; Polymer Science |
| DOI | 10.1016/j.carbpol.2012.11.028 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Chemistry ; Polymer Science |
| WOS类目 | Chemistry, Applied ; Chemistry, Organic ; Polymer Science |
| WOS记录号 | WOS:000315616900099 |
| WOS关键词 | BIOLOGICAL-ACTIVITIES ; SCAVENGING ACTIVITY ; CHITIN DEACETYLASE ; IN-VITRO ; CHITOSAN ; CHITOOLIGOSACCHARIDES ; OLIGOSACCHARIDES ; OLIGOMERS ; CHITIN/CHITOSAN ; HYDROLYSIS |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/16714 |
| 专题 | 海洋生物技术研发中心 |
| 通讯作者 | Xing, RG |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Li, Kecheng,Liu, Song,Xing, Ronge,et al. Preparation, characterization and antioxidant activity of two partially N-acetylated chitotrioses[J]. CARBOHYDRATE POLYMERS,2013,92(2):1730-1736. |
| APA | Li, Kecheng,Liu, Song,Xing, Ronge,Qin, Yukun,Li, Pengcheng,&Xing, RG.(2013).Preparation, characterization and antioxidant activity of two partially N-acetylated chitotrioses.CARBOHYDRATE POLYMERS,92(2),1730-1736. |
| MLA | Li, Kecheng,et al."Preparation, characterization and antioxidant activity of two partially N-acetylated chitotrioses".CARBOHYDRATE POLYMERS 92.2(2013):1730-1736. |
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