Genome and Transcriptome Analyses Provide Insight into the Euryhaline Adaptation Mechanism of Crassostrea gigas

doi:10.1371/journal.pone.0058563

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	Genome and Transcriptome Analyses Provide Insight into the Euryhaline Adaptation Mechanism of Crassostrea gigas
	Meng, Jie1,2 ; Zhu, Qihui1,2 ; Zhang, Linlin 1; Li, Chunyan1,2 ; Li, Li 1; She, Zhicai1,2 ; Huang, Baoyu1,2 ; Zhang, Guofan 1; Li, L
	2013-03-12
发表期刊	PLOS ONE
ISSN	1932-6203
卷号	8 期号:3 页码:e58563
文章类型	Article
摘要	Background: The Pacific oyster, Crassostrea gigas, has developed special mechanisms to regulate its osmotic balance to adapt to fluctuations of salinities in coastal zones. To understand the oyster's euryhaline adaptation, we analyzed salt stress effectors metabolism pathways under different salinities (salt 5, 10, 15, 20, 25, 30 and 40 for 7 days) using transcriptome data, physiology experiment and quantitative real-time PCR.; Background: The Pacific oyster, Crassostrea gigas, has developed special mechanisms to regulate its osmotic balance to adapt to fluctuations of salinities in coastal zones. To understand the oyster's euryhaline adaptation, we analyzed salt stress effectors metabolism pathways under different salinities (salt 5, 10, 15, 20, 25, 30 and 40 for 7 days) using transcriptome data, physiology experiment and quantitative real-time PCR. Results: Transcriptome data uncovered 189, 480, 207 and 80 marker genes for monitoring physiology status of oysters and the environment conditions. Three known salt stress effectors (involving ion channels, aquaporins and free amino acids) were examined. The analysis of ion channels and aquaporins indicated that 7 days long-term salt stress inhibited voltage-gated Na+/K+ channel and aquaporin but increased calcium-activated K+ channel and Ca2+ channel. As the most important category of osmotic stress effector, we analyzed the oyster FAAs metabolism pathways (including taurine, glycine, alanine, beta-alanine, proline and arginine) and explained FAAs functional mechanism for oyster low salinity adaptation. FAAs metabolism key enzyme genes displayed expression differentiation in low salinity adapted individuals comparing with control which further indicated that FAAs played important roles for oyster salinity adaptation. A global metabolic pathway analysis (iPath) of oyster expanded genes displayed a co-expansion of FAAs metabolism in C. gigas compared with seven other species, suggesting oyster's powerful ability regarding FAAs metabolism, allowing it to adapt to fluctuating salinities, which may be one important mechanism underlying euryhaline adaption in oyster. Additionally, using transcriptome data analysis, we uncovered salt stress transduction networks in C. gigas. Conclusions: Our results represented oyster salt stress effectors functional mechanisms under salt stress conditions and explained the expansion of FAAs metabolism pathways as the most important effectors for oyster euryhaline adaptation. This study was the first to explain oyster euryhaline adaptation at a genome-wide scale in C. gigas.
学科领域	Science & Technology - Other Topics
DOI	10.1371/journal.pone.0058563
URL	查看原文
收录类别	SCI
语种	英语
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000316252500030
WOS关键词	CELL-VOLUME REGULATION ; PACIFIC OYSTER ; AMINO-ACID ; INTERTIDAL ZONE ; GENE-EXPRESSION ; MARINE MOLLUSKS ; MARKER GENES ; SALINITY ; STRESS ; TAURINE
WOS标题词	Science & Technology
引用统计	被引频次：149[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/16701
专题	海洋生物技术研发中心实验海洋生物学重点实验室
通讯作者	Li, L
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Meng, Jie,Zhu, Qihui,Zhang, Linlin,et al. Genome and Transcriptome Analyses Provide Insight into the Euryhaline Adaptation Mechanism of Crassostrea gigas[J]. PLOS ONE,2013,8(3):e58563.
APA	Meng, Jie.,Zhu, Qihui.,Zhang, Linlin.,Li, Chunyan.,Li, Li.,...&Li, L.(2013).Genome and Transcriptome Analyses Provide Insight into the Euryhaline Adaptation Mechanism of Crassostrea gigas.PLOS ONE,8(3),e58563.
MLA	Meng, Jie,et al."Genome and Transcriptome Analyses Provide Insight into the Euryhaline Adaptation Mechanism of Crassostrea gigas".PLOS ONE 8.3(2013):e58563.

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