Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| The polymorphism in the promoter region of metallothionein 1 is associated with heat tolerance of scallop Argopecten irradians | |
Yang, Chuanyan1; Wang, Lingling1; Jiang, Qiufen1,2; Wang, Jingjing1,2 ; Yue, Feng1,2; Zhang, Huan1; Sun, Zhibin1; Song, Linsheng1; Song, LS
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| 2013-09-10 | |
| 发表期刊 | GENE
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| ISSN | 0378-1119 |
| 卷号 | 526期号:2页码:429-436 |
| 文章类型 | Article |
| 摘要 | Metallothioneins (MTs), a superfamily of cysteine-rich proteins, perform multiple functions, such as maintaining homeostasis of essential metals, detoxification of toxic metals and scavenging of oxyradicals. In this study, the promoter region of a metallothionein (MT) gene from Bay scallop Argopecten irradians (designed as AiMT1) was cloned by the technique of genomic DNA walking, and the polymorphisms in this region were screened to find their association with susceptibility or tolerance to high temperature stress. One insert-deletion (ins-del) polymorphism and sixteen single nucleotide polymorphisms (SNPs) were identified in the amplified promoter region. Two SNPs, -375 T-C and -337 A-C, were selected to analyze their distribution in the two Bay scallop populations collected from southern and northern China coast, which were identified as heat resistant and heat susceptible stocks, respectively. There were three genotypes, T/T, T/C and C/C, at locus -375, and their frequencies were 25%, 61.1% and 13.9% in the heat susceptible stock, while 34.2%, 42.1% and 23.7% in the resistant stock, respectively. There was no significant difference in the frequency distribution of different genotypes between the two stocks (P > 0.05). In contrast, at locus -337, three genotypes A/A, A/C and C/C were revealed with the frequencies of 11.6%, 34.9% and 53.5% in the heat susceptible stock, while 45.7%, 32.6% and 21.7% in the heat resistant stock, respectively. The frequency of C/C genotype in the heat susceptible stock was significantly higher (P < 0.01) than that in the heat resistant stock, while the frequency of A/A in the heat resistant stock was significantly higher (P < 0.01) than that in the heat susceptible stock. Furthermore, the expression of AiMT1 mRNA in scallops with C/C genotype was significantly higher than that with A/A genotype (P < 0.05) after an acute heat treatment at 28 degrees C for 120 min. These results implied that the polymorphism at locus -337 of AiMT1 was associated with the susceptibility/tolerance of scallops to heat stress, and the -337 A/A genotype could be a potential marker available in future selection of Bay scallop with heat tolerance. (C) 2013 Elsevier B.V. All rights reserved.; Metallothioneins (MTs), a superfamily of cysteine-rich proteins, perform multiple functions, such as maintaining homeostasis of essential metals, detoxification of toxic metals and scavenging of oxyradicals. In this study, the promoter region of a metallothionein (MT) gene from Bay scallop Argopecten irradians (designed as AiMT1) was cloned by the technique of genomic DNA walking, and the polymorphisms in this region were screened to find their association with susceptibility or tolerance to high temperature stress. One insert-deletion (ins-del) polymorphism and sixteen single nucleotide polymorphisms (SNPs) were identified in the amplified promoter region. Two SNPs, -375 T-C and -337 A-C, were selected to analyze their distribution in the two Bay scallop populations collected from southern and northern China coast, which were identified as heat resistant and heat susceptible stocks, respectively. There were three genotypes, T/T, T/C and C/C, at locus -375, and their frequencies were 25%, 61.1% and 13.9% in the heat susceptible stock, while 34.2%, 42.1% and 23.7% in the resistant stock, respectively. There was no significant difference in the frequency distribution of different genotypes between the two stocks (P > 0.05). In contrast, at locus -337, three genotypes A/A, A/C and C/C were revealed with the frequencies of 11.6%, 34.9% and 53.5% in the heat susceptible stock, while 45.7%, 32.6% and 21.7% in the heat resistant stock, respectively. The frequency of C/C genotype in the heat susceptible stock was significantly higher (P < 0.01) than that in the heat resistant stock, while the frequency of A/A in the heat resistant stock was significantly higher (P < 0.01) than that in the heat susceptible stock. Furthermore, the expression of AiMT1 mRNA in scallops with C/C genotype was significantly higher than that with A/A genotype (P < 0.05) after an acute heat treatment at 28 degrees C for 120 min. These results implied that the polymorphism at locus -337 of AiMT1 was associated with the susceptibility/tolerance of scallops to heat stress, and the -337 A/A genotype could be a potential marker available in future selection of Bay scallop with heat tolerance. (C) 2013 Elsevier B.V. All rights reserved. |
| 关键词 | Argopecten Irradians Metallothionein 1 Promoter Single Nucleotide Polymorphism Heat Tolerance |
| 学科领域 | Genetics & Heredity |
| DOI | 10.1016/j.gene.2013.05.033 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Genetics & Heredity |
| WOS类目 | Genetics & Heredity |
| WOS记录号 | WOS:000323396500049 |
| WOS关键词 | METAL REGULATORY ELEMENTS ; OXIDATIVE STRESS ; DIABETES-MELLITUS ; MESSENGER-RNA ; GENE PROMOTER ; BAY SCALLOP ; DNA-DAMAGE ; EXPRESSION ; FISH ; COMPLICATIONS |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/16657 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Song, LS |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 第一作者单位 | 实验海洋生物学重点实验室 |
| 推荐引用方式 GB/T 7714 | Yang, Chuanyan,Wang, Lingling,Jiang, Qiufen,et al. The polymorphism in the promoter region of metallothionein 1 is associated with heat tolerance of scallop Argopecten irradians[J]. GENE,2013,526(2):429-436. |
| APA | Yang, Chuanyan.,Wang, Lingling.,Jiang, Qiufen.,Wang, Jingjing.,Yue, Feng.,...&Song, LS.(2013).The polymorphism in the promoter region of metallothionein 1 is associated with heat tolerance of scallop Argopecten irradians.GENE,526(2),429-436. |
| MLA | Yang, Chuanyan,et al."The polymorphism in the promoter region of metallothionein 1 is associated with heat tolerance of scallop Argopecten irradians".GENE 526.2(2013):429-436. |
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