Phenotypic characterization, virulence, and immunogenicity of Edwardsiella tarda LSE40 aroA mutant

doi:10.1007/s00253-013-4813-1

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	Phenotypic characterization, virulence, and immunogenicity of Edwardsiella tarda LSE40 aroA mutant
	Mo, Zhao-Lan 1; Li, Jie1 ; Li, Gui-Yang 1; Xiao, Peng 2; Mo, ZL
	2013-07-01
发表期刊	APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
ISSN	0175-7598
卷号	97 期号:14 页码:6325-6335
文章类型	Article
摘要	Bacterial aro mutants are frequently used as live attenuated vaccines for domestic animals. In this study, we characterized Edwardsiella tarda strain LSE40 with a deletion in the aroA gene. In addition to autotrophy, the aroA mutant appeared to have delayed cell division and reductions in its swarming motility, biofilm formation, and production of translocator proteins in the type III secretory system. The mutant exhibited high virulence attenuation in turbot fish, Scophthalmus maximus (L.), where the 50 % lethal dose increased by more than 3 log(10) via intraperitoneal (i.p.) injection and by > 2 log(10) via immersion exposure compared with the wild-type parent strain. A tissue persistence study showed that the mutant retained the ability to invade and spread in turbot and viable cells could be detected up to 28 days after i.p. infection and 21 days after immersion exposure. These results suggested a pleiotropic role for aroA in the physiological behavior of E. tarda. Turbot exhibited a good humoral response and the enhanced expression of innate immune factors, interleukin 1 beta and lysozyme, when vaccinated with aroA mutant at 10(5) CFU via i.p. injection and at 10(8) CFU via immersion exposure. However, the aroA mutant did not provide effective protection for turbot against edwardsiellosis following i.p. vaccination at doses of 10(4)-10(6) CFU or immersion vaccination at doses of 10(6)-10(8) CFU ml(-1). We hypothesized that the aroA mutant did not trigger an appropriate T cell-immune response in turbot against infection of E. tarda.; Bacterial aro mutants are frequently used as live attenuated vaccines for domestic animals. In this study, we characterized Edwardsiella tarda strain LSE40 with a deletion in the aroA gene. In addition to autotrophy, the aroA mutant appeared to have delayed cell division and reductions in its swarming motility, biofilm formation, and production of translocator proteins in the type III secretory system. The mutant exhibited high virulence attenuation in turbot fish, Scophthalmus maximus (L.), where the 50 % lethal dose increased by more than 3 log(10) via intraperitoneal (i.p.) injection and by > 2 log(10) via immersion exposure compared with the wild-type parent strain. A tissue persistence study showed that the mutant retained the ability to invade and spread in turbot and viable cells could be detected up to 28 days after i.p. infection and 21 days after immersion exposure. These results suggested a pleiotropic role for aroA in the physiological behavior of E. tarda. Turbot exhibited a good humoral response and the enhanced expression of innate immune factors, interleukin 1 beta and lysozyme, when vaccinated with aroA mutant at 10(5) CFU via i.p. injection and at 10(8) CFU via immersion exposure. However, the aroA mutant did not provide effective protection for turbot against edwardsiellosis following i.p. vaccination at doses of 10(4)-10(6) CFU or immersion vaccination at doses of 10(6)-10(8) CFU ml(-1). We hypothesized that the aroA mutant did not trigger an appropriate T cell-immune response in turbot against infection of E. tarda.
关键词	Edwardsiella Tarda Aroa Phenotype Protein Secretion Virulence Immunogenecity
学科领域	Biotechnology & Applied Microbiology
DOI	10.1007/s00253-013-4813-1
URL	查看原文
收录类别	SCI
语种	英语
WOS研究方向	Biotechnology & Applied Microbiology
WOS类目	Biotechnology & Applied Microbiology
WOS记录号	WOS:000321121800020
WOS关键词	III SECRETION SYSTEM ; SALMONELLA-TYPHIMURIUM ; AEROMONAS-SALMONICIDA ; ATTENUATED VACCINE ; BIOFILM FORMATION ; FISH ; LIVE ; IDENTIFICATION ; GENES ; FURUNCULOSIS
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/16612
专题	实验海洋生物学重点实验室
通讯作者	Mo, ZL
作者单位	1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Mo, Zhao-Lan,Li, Jie,Li, Gui-Yang,et al. Phenotypic characterization, virulence, and immunogenicity of Edwardsiella tarda LSE40 aroA mutant[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2013,97(14):6325-6335.
APA	Mo, Zhao-Lan,Li, Jie,Li, Gui-Yang,Xiao, Peng,&Mo, ZL.(2013).Phenotypic characterization, virulence, and immunogenicity of Edwardsiella tarda LSE40 aroA mutant.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,97(14),6325-6335.
MLA	Mo, Zhao-Lan,et al."Phenotypic characterization, virulence, and immunogenicity of Edwardsiella tarda LSE40 aroA mutant".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 97.14(2013):6325-6335.

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