Pigment profiling of 'QN Orange'scallop and 'Bohai Red' scallop adductor muscles by liquid chromatography coupled with triple quadrupole mass spectrometry

doi:10.1016/j.aquaculture.2019.04.062

IOCAS-IR > 实验海洋生物学重点实验室

	Pigment profiling of 'QN Orange'scallop and 'Bohai Red' scallop adductor muscles by liquid chromatography coupled with triple quadrupole mass spectrometry
	Zhao, Qian 1; Chen, Juanjuan 1; Zhu, Si 1; Liu, Xiao 2; Ma, Bin 3; Wang, Chunde 4; Xu, Jilin 1; Yan, Xiaojun 1
	2019-06-15
发表期刊	AQUACULTURE
ISSN	0044-8486
卷号	508 页码:178-185
通讯作者	Chen, Juanjuan([email protected]) ; Xu, Jilin([email protected])
摘要	Two new scallop strains, the 'QN Orange' scallop and the 'Bohai Red' scallop, were selected from the hybrid cohort between the bay scallop (Argopecten irradians irradians) and the Peruvian scallop (Argopecten purpuratus) to improve the bay scallop stock, which has suffered from significant inbreeding depression characterized by poor growth and high mortality. Although both strains were originated from the same hybrid cohort, the 'QN Orange' scallops possess orange adductor muscles while the 'Bohai Red' scallops have white adductor muscles. To explore the pigment profiles of adductor muscle coloration in these two scallop strains, an optimized method based on liquid chromatography coupled with triple quadrupole mass spectrometry (HPLC-QqQ-MS) with atmospheric pressure chemical ionization (APCI) was established to determine chlorophyll (chlorophyll a and chlorophyll b) and carotenoids (including alpha-carotene, beta-carotene, epsilon-carotene, gamma-carotene, zeta-carotene, lutein, fucoxanthin, zeaxanthin, canthaxanthin, pectenoxanthin, and pectenolone). The results showed that the total contents of carotenoids in the adductor muscle, gill, and mantle of 'QN Orange' scallops were 82.68, 105.49, and 100.49 mu g g(-1), which were 49.88, 3.26, and 4.18 times higher than those in the 'Bohai Red' scallops, respectively. In particular, the contents of the pigments pectenolone, pectenoxanthin, fucoxanthin, zeaxanthin, and lutein in the 'QN Orange' scallops were 171.58, 61.67, 3.87, 22.16 and 23.27 mu g g(-1), which were 6.92, 3.38, 27.64, 15.75, and 13.00 times higher than those in the 'Bohai Red' scallops, respectively. These results suggest that the orange coloration in the 'QN Orange' scallops might be caused by the accumulation of carotenoids.
关键词	Argopecten irradians irradians Argopecten purpuratus 'QN Orange'scallop 'Bohai Red' scallop HPLC-MS Carotenoid accumulation
DOI	10.1016/j.aquaculture.2019.04.062
收录类别	SCI
语种	英语
资助项目	National Key R&D Program of China[2018YFD0901103]###2690; National Natural Science Foundation of China[41676152]###1167; National Natural Science Foundation of China[31572618]###1883; Earmarked Fund for Modern Agroindustry Technology Research System, China[CARS-49]###2691; K. C. Wong Magna Fund in Ningbo University###426; National Key R&D Program of China[2018YFD0901103]; National Natural Science Foundation of China[41676152]; National Natural Science Foundation of China[31572618]; Earmarked Fund for Modern Agroindustry Technology Research System, China[CARS-49]; K. C. WONG Magna Fund in Ningbo University
WOS研究方向	Fisheries ; Marine & Freshwater Biology
WOS类目	Fisheries ; Marine & Freshwater Biology
WOS记录号	WOS:000469922700021
出版者	ELSEVIER SCIENCE BV
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/161608
专题	实验海洋生物学重点实验室
通讯作者	Chen, Juanjuan; Xu, Jilin
作者单位	1.Ningbo Univ, Key Lab Marine Biotechnol Zhejiang Prov, Ningbo 315211, Zhejiang, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China 3.Qingdao Oceanwide BioTech Co Ltd, Qingdao 266101, Shandong, Peoples R China 4.Qingdao Agr Univ, Qingdao 266109, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Qian,Chen, Juanjuan,Zhu, Si,et al. Pigment profiling of 'QN Orange'scallop and 'Bohai Red' scallop adductor muscles by liquid chromatography coupled with triple quadrupole mass spectrometry[J]. AQUACULTURE,2019,508:178-185.
APA	Zhao, Qian.,Chen, Juanjuan.,Zhu, Si.,Liu, Xiao.,Ma, Bin.,...&Yan, Xiaojun.(2019).Pigment profiling of 'QN Orange'scallop and 'Bohai Red' scallop adductor muscles by liquid chromatography coupled with triple quadrupole mass spectrometry.AQUACULTURE,508,178-185.
MLA	Zhao, Qian,et al."Pigment profiling of 'QN Orange'scallop and 'Bohai Red' scallop adductor muscles by liquid chromatography coupled with triple quadrupole mass spectrometry".AQUACULTURE 508(2019):178-185.