Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Comparative transcriptome analysis explores maternal to zygotic transition during Eriocheir sinensis early embryogenesis | |
Ning, Junhao1,2,3,4; Song, Chengwen1,2,3 ; Luo, Danli1,3,4; Liu, Yuan1,2,3; Liu, Hourong1,3,4; Cui, Zhaoxia1,2,3
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| 2019-02-15 | |
| 发表期刊 | GENE
 |
| ISSN | 0378-1119 |
| 卷号 | 685页码:12-20 |
| 通讯作者 | Cui, Zhaoxia([email protected]) |
| 摘要 | The maternal genome directs almost all aspects of early animal development. As development proceeds, the elimination of maternal gene products and zygotic genome activation (ZGA) occur during the maternal to zygotic transition (MZT). To study the molecular mechanisms regulating this developmental event in Eriocheir sinensis, RNA-Seq technology was applied to generate comprehensive information on transcriptome dynamics during early embryonic stages. In total, 32,088 annotated unigenes were obtained from the transcriptomes of fertilized eggs and embryos at the cleavage (2-4 cell) and blastula stage. A total of 566 maternal genes and 1165 zygotic genes were isolated, among which 103 and 266 genes were predicted conserved maternal transcripts (COMATs) and conserved zygotic transcripts (COZYTs), respectively. The COMATs performed housekeeping gene functions and may be essential for initiating early embryogenesis of the Bilateria. Furthermore, 87, 76 and 117 differentially expressed genes associated with the MZT, morphogenesis and immunity were identified when compared the three transcriptomic datasets. We also unmask that the MZT takes place around the cleavage stage, when the genes involved in the clearance of maternal gene products and the ZGA were significantly up-regulated. Taken together, these datasets provide a valuable resource for understanding the mechanisms of early developmental events in E. sinensis, and facilitate further studies on molecular mechanisms of asynchronous development in crabs. |
| 关键词 | Mitten crabs Embryogenesis Maternal to zygotic transition Transcriptomics |
| DOI | 10.1016/j.gene.2018.10.036 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | Natural Science Foundation of Shandong Province[ZR2017QD001]###2412; Scientific and Technological Innovation Project of Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02]###1178; Natural Science Foundation of Shandong Province[ZR2017QD001]; Scientific and Technological Innovation Project of Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02] |
| WOS研究方向 | Genetics & Heredity |
| WOS类目 | Genetics & Heredity |
| WOS记录号 | WOS:000455692300002 |
| 出版者 | ELSEVIER SCIENCE BV |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/160707 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Cui, Zhaoxia |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 通讯作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Ning, Junhao,Song, Chengwen,Luo, Danli,et al. Comparative transcriptome analysis explores maternal to zygotic transition during Eriocheir sinensis early embryogenesis[J]. GENE,2019,685:12-20. |
| APA | Ning, Junhao,Song, Chengwen,Luo, Danli,Liu, Yuan,Liu, Hourong,&Cui, Zhaoxia.(2019).Comparative transcriptome analysis explores maternal to zygotic transition during Eriocheir sinensis early embryogenesis.GENE,685,12-20. |
| MLA | Ning, Junhao,et al."Comparative transcriptome analysis explores maternal to zygotic transition during Eriocheir sinensis early embryogenesis".GENE 685(2019):12-20. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Comparative transcri（2005KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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