Knowledge Management System Of Institute of Oceanology, Chinese Academy of Sciences
| Construction of a high-density genetic map and fine QTL mapping for growth and nutritional traits of Crassostrea gigas | |
Li, Chunyan1,2,3,5,6 ; Wang, Jinpeng1,2,3,5,6; Song, Kai1,3,5,6; Meng, Jie1,3,5,6; Xu, Fei1,3,5,6; Li, Li1,4,5,6; Zhang, Guofan1,3,5,6
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| 2018-08-22 | |
| 发表期刊 | BMC GENOMICS
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| ISSN | 1471-2164 |
| 卷号 | 19页码:15 |
| 通讯作者 | Li, Li([email protected]) ; Zhang, Guofan([email protected]) |
| 摘要 | Background: Both growth and nutritional traits are important economic traits of Crassostrea gigas (C. gigas) in industry. But few work has been done to study the genetic architecture of nutritional traits of the oyster. In this study, we constructed a high-density genetic map of C. gigas to help assemble the genome sequence onto chromosomes, meanwhile explore the genetic basis for nutritional traits via quantitative trait loci (QTL) mapping. Results: The constructed genetic map contained 5024 evenly distributed markers, with an average marker interval of 0.68 cM, thus representing the densest genetic map produced for the oyster. According to the high collinearity between the consensus map and the oyster genome, 1574 scaffold (about 70%) of the genome sequence of C. gigas were successfully anchored to 10 linkage groups (LGs) of the consensus map. Using this high-qualified genetic map, we then conducted QTL analysis for growth and nutritional traits, the latter of which includes glycogen, amino acid (AA), and fatty acid (FA). Overall, 41 QTLs were detected for 17 traits. In addition, six candidate genes identified in the QTL interval showed significant correlation with the traits on transcriptional levels. These genes include growth-related genes AMY and BMP1, AA metabolism related genes PLSCR and GR, and FA metabolism regulation genes DYRK and ADAMTS. Conclusion: Using the constructed high-qualified linkage map, this study not only assembled nearly 70% of the oyster genome sequence onto chromosomes, but also identified valuable markers and candidate genes for growth and nutritional traits, especially for AA and FA that undergone few studies before. These findings will facilitate genome assembly and molecular breeding of important economic traits in C. gigas. |
| 关键词 | Crassostrea gigas Genetic map Collinearity Quantitative trait loci (QTL) mapping Growth Nutrition |
| DOI | 10.1186/s12864-018-4996-z |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | National Natural Science Foundation of China[31530079]###2; National Natural Science Foundation of China[31572620]###898; Strategic Priority Research Program of Western Pacific Ocean System: Structure, Dynamics and Consequences[XDA586 11020305]###2136; earmarked fund for Modern Agro-industry Technology Research System[CARS-49]###157; National Natural Science Foundation of China[31530079]; National Natural Science Foundation of China[31572620]; Strategic Priority Research Program of Western Pacific Ocean System: Structure, Dynamics and Consequences[XDA586 11020305]; Earmarked Fund for Modern Agro-industry Technology Research System[CARS-49] |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Genetics & Heredity |
| WOS类目 | Biotechnology & Applied Microbiology ; Genetics & Heredity |
| WOS记录号 | WOS:000442532000005 |
| 出版者 | BMC |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/159887 |
| 专题 | 中国科学院海洋研究所 |
| 通讯作者 | Li, Li; Zhang, Guofan |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries & Aquaculture, Qingdao, Peoples R China 5.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China 6.Chinese Acad Sci, Inst Oceanol, Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 通讯作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Li, Chunyan,Wang, Jinpeng,Song, Kai,et al. Construction of a high-density genetic map and fine QTL mapping for growth and nutritional traits of Crassostrea gigas[J]. BMC GENOMICS,2018,19:15. |
| APA | Li, Chunyan.,Wang, Jinpeng.,Song, Kai.,Meng, Jie.,Xu, Fei.,...&Zhang, Guofan.(2018).Construction of a high-density genetic map and fine QTL mapping for growth and nutritional traits of Crassostrea gigas.BMC GENOMICS,19,15. |
| MLA | Li, Chunyan,et al."Construction of a high-density genetic map and fine QTL mapping for growth and nutritional traits of Crassostrea gigas".BMC GENOMICS 19(2018):15. |
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