实验海洋生物学重点实验室知识库

QQ客服 官方微博 反馈留言

	Sequence features, expression profiles and biochemical characteristics of a sigma class glutathione S-transferase gene (AiGST sigma) from bay scallop Argopecten irradians
	Wang, M.1,3; Wang, B.1; Liu, M.1; Jiang, K.1; Wang, L.1,2
	2018
发表期刊	ISJ-INVERTEBRATE SURVIVAL JOURNAL
ISSN	1824-307X
卷号	15页码:19-30
通讯作者	Wang, L.([email protected])
摘要	Glutathione S-transferases (GSTs) are a class of enzymes that facilitate the detoxification of xenobiotics and also play important roles in innate immunity. In the present study, a novel sigma class GST gene (designated as AiGST sigma) was cloned from the bay scallop Argopecten irradians via rapid amplification of cDNA ends (RACE) technique. The complete cDNA sequence of AiGSTs consisted of a 5' untranslated regions (UTR) of 48 bp, a 3' UTR of 113 bp with a poly A tail and an open reading frame (ORF) of 618 bp. The ORF encoded a polypeptide of 205 amino acid residues with a calculated molecular mass of approximately 23.11 kDa and a theoretical isoelectric point of 5.354. The deduced amino acid sequence of AiGSTs contained a GST_N domain and a GST_C domain, and exhibited high similarity with other reported sigma class GSTs. In the phylogenetic tree, AiGSTs was located in the sigma class GSTs sub-branch. The AiGSTs mRNA transcripts were constitutively expressed in the tissues of hemocytes, muscle, mantle, gill, hepatopancreas and gonad, with the highest expression level in hemocytes, and the mRNA expression levels of AiGSTs were significantly up-regulated in hemocytes after various pathogen associated molecular patterns (PAMPs) stimulation. The purified recombinant AiGSTs protein exhibited catalytic activity against the common substrate 1-chloro-2, 4-dinitrobenzene (CDNB) with low thermal stability and narrow optimum pH spectrum. All these results indicated that AiGSTs was a fragile but efficient antioxidant enzyme and was potentially involved in the innate immune responses of scallop.
关键词	Argopecten irradians glutathione S-transferase innate immunity
收录类别	SCI
语种	英语
资助项目	Key Research Program of the Chinese Academy of Sciences[KFZD-SW-106]###819; Key Research Program of the Chinese Academy of Sciences[KFZD-SW-106]
WOS研究方向	Immunology ; Zoology
WOS类目	Immunology ; Zoology
WOS记录号	WOS:000423724700004
出版者	INVERTEBRATE SURVIVAL JOURNAL
引用统计	被引频次：4[WOS]   [WOS记录]     [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/157993
专题	实验海洋生物学重点实验室
通讯作者	Wang, L.
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Res Platform Marine Mol Biotechnol, Qingdao 266237, Peoples R China
第一作者单位	实验海洋生物学重点实验室
通讯作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Wang, M.,Wang, B.,Liu, M.,et al. Sequence features, expression profiles and biochemical characteristics of a sigma class glutathione S-transferase gene (AiGST sigma) from bay scallop Argopecten irradians[J]. ISJ-INVERTEBRATE SURVIVAL JOURNAL,2018,15:19-30.
APA	Wang, M.,Wang, B.,Liu, M.,Jiang, K.,&Wang, L..(2018).Sequence features, expression profiles and biochemical characteristics of a sigma class glutathione S-transferase gene (AiGST sigma) from bay scallop Argopecten irradians.ISJ-INVERTEBRATE SURVIVAL JOURNAL,15,19-30.
MLA	Wang, M.,et al."Sequence features, expression profiles and biochemical characteristics of a sigma class glutathione S-transferase gene (AiGST sigma) from bay scallop Argopecten irradians".ISJ-INVERTEBRATE SURVIVAL JOURNAL 15(2018):19-30.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Sequence features, e（882KB）	期刊论文	出版稿	限制开放	CC BY-NC-SA	浏览

个性服务

推荐该条目

保存到收藏夹

查看访问统计

导出为Endnote文件

谷歌学术

谷歌学术中相似的文章

[Wang, M.]的文章

[Wang, B.]的文章

[Liu, M.]的文章

百度学术

百度学术中相似的文章

[Wang, M.]的文章

[Wang, B.]的文章

[Liu, M.]的文章

必应学术

必应学术中相似的文章

[Wang, M.]的文章

[Wang, B.]的文章

[Liu, M.]的文章

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。