Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Phosphoproteomic analyses of kidneys of Atlantic salmon infected with Aeromonas salmonicida | |
Liu, Peng-fei1,2,3; Du, Yishuai1,2,3; Meng, Lingjie1,2,3 ; Li, Xian1,2,3; Yang, Dung5; Liu, Ying4
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| 2019-02-14 | |
| 发表期刊 | SCIENTIFIC REPORTS
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| ISSN | 2045-2322 |
| 卷号 | 9页码:12 |
| 通讯作者 | Yang, Dung([email protected]) ; Liu, Ying([email protected]) |
| 摘要 | Aeromonas salmonicida (A. salmonicida) is a pathogenic bacterium that causes furunculosis and poses a significant global risk, particularly in economic activities such as Atlantic salmon (Salmo salar) farming. In a previous study, we identified proteins that are significantly upregulated in kidneys of Atlantic salmon challenged with A. salmonicida. Phosphoproteomic analyses were conducted to further clarify the dynamic changes in protein phosphorylation patterns triggered by bacterial infection. To our knowledge, this is the first study to characterize phosphorylation events in proteins from A. salmonicida-infected Atlantic salmon. Overall, we identified over 5635 phosphorylation sites in 3112 proteins, and 1502 up-regulated and 77 down-regulated proteins quantified as a 1.5-fold or greater change relative to control levels. Based on the combined data from proteomic and motif analyses, we hypothesize that five prospective novel kinases (VRK3, GAK, HCK, PKCd and RSK6) with common functions in inflammatory processes and cellular pathways to regulate apoptosis and the cytoskeleton could serve as potential biomarkers against bacterial propagation in fish. Data from STRING-based functional network analyses indicate that fga is the most central protein. Our collective findings provide new insights into protein phosphorylation patterns, which may serve as effective indicators of A. salmonicida infection in Atlantic salmon. |
| DOI | 10.1038/s41598-019-38890-3 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | Innovation project[16CXZ027]###482; State Key Laboratory of Proteomics[SKLP-O201507]###481; State Key Laboratory of Proteomics[SKLP-O201704]###480; Beijing Nova Program[Z161100004916148]###479; Chinese State Key Projects for Basic Research (973 Program)[2014CBA02001]###478; Chinese State Key Projects for Basic Research (973 Program)[2015CB910700]###477; National Natural Science Foundation of China[31671376]###476; National Key R&D Program of China[2017YFD0701700]###440; National Key R&D Program of China[2017YFD0701700]; National Natural Science Foundation of China[31671376]; Chinese State Key Projects for Basic Research (973 Program)[2015CB910700]; Chinese State Key Projects for Basic Research (973 Program)[2014CBA02001]; Beijing Nova Program[Z161100004916148]; State Key Laboratory of Proteomics[SKLP-O201704]; State Key Laboratory of Proteomics[SKLP-O201507]; Innovation project[16CXZ027] |
| WOS研究方向 | Science & Technology - Other Topics |
| WOS类目 | Multidisciplinary Sciences |
| WOS记录号 | WOS:000458619600100 |
| 出版者 | NATURE PUBLISHING GROUP |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/155557 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Yang, Dung; Liu, Ying |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266235, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 4.Dalian Ocean Univ, Dalian, Peoples R China 5.Natl Ctr Prot Sci Beijing, State Key Lab Prote, Beijing Proteome Res Ctr, Beijing Inst Life, Beijing 102206, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Liu, Peng-fei,Du, Yishuai,Meng, Lingjie,et al. Phosphoproteomic analyses of kidneys of Atlantic salmon infected with Aeromonas salmonicida[J]. SCIENTIFIC REPORTS,2019,9:12. |
| APA | Liu, Peng-fei,Du, Yishuai,Meng, Lingjie,Li, Xian,Yang, Dung,&Liu, Ying.(2019).Phosphoproteomic analyses of kidneys of Atlantic salmon infected with Aeromonas salmonicida.SCIENTIFIC REPORTS,9,12. |
| MLA | Liu, Peng-fei,et al."Phosphoproteomic analyses of kidneys of Atlantic salmon infected with Aeromonas salmonicida".SCIENTIFIC REPORTS 9(2019):12. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| s41598-019-38890-3.p（2753KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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