Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Insights into individual variations in nematocyst venoms from the giant jellyfish Nemopilema nomurai in the Yellow Sea | |
Yue, Yang1,3 ; Yu, Huahua1,2,3; Li, Rongfeng1,2,3; Liu, Song1,2,3; Xing, Ronge1,2,3; Li, Pengcheng1,2,3
| |
| 2019-03-04 | |
| 发表期刊 | SCIENTIFIC REPORTS
 |
| ISSN | 2045-2322 |
| 卷号 | 9页码:13 |
| 通讯作者 | Yu, Huahua([email protected]) ; Li, Pengcheng([email protected]) |
| 摘要 | The giant jellyfish, Nemopilema nomurai, is widely distributed from the Eastern China Sea to the northern part of the Yellow Sea and has resulted in numerous hospitalizations in coastal areas of China, especially in Northern China. Our previous studies have revealed sting-related proteins in the venom of the jellyfish N. nomurai by using experimental and omics-based approaches; however, the variable symptoms of patients who have been stung by N. nomurai are not fully understood. This limited knowledge led to an examination of whether intraspecific variations occur in the venom of different N. nomurai. In the present study, 13 specimens of N. nomurai were collected from the Yellow Sea, and their venom was characterized by profiling differences in biochemical properties and biological activities. SDS-PAGE analysis presented recognizable differences in the number, intensity and presence of some protein bands. Moreover, enzymatic assays revealed considerable quantitative variations in metalloproteinase activity and PLA2-like activity. In particular, zymography assays of proteases demonstrated the general presence of abundant metalloproteinases in jellyfish nematocyst venom; however, the catalytic activities varied greatly among some specific metalloproteinases in the 28-46 kDa or 57-83 kDa range. Hemolytic assays using sheep erythrocytes suggested a predominant variance in the toxicities of different individual jellyfish venoms, with the difference between the most hemolytic and the least hemolytic venom as large as 77-fold. The current data suggested remarkable variations in the nematocyst venoms of individual N. nomurai jellyfish. These observations will provide a new understanding of the clinical manifestations induced by N. nomurai jellyfish stings and will therefore have important implications for preventing and treating jellyfish envenomations. |
| DOI | 10.1038/s41598-019-40109-4 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | Qingdao Science and Technology Project[17-3-3-21-nsh]###213; Key Research and Development Plan of Shandong Province[2017GHY215003]###212; National Key RD Plan[2017YFE0111100-04]###211; National Natural Science Foundation of China[41406152]###210; National Natural Science Foundation of China[41376004]###209; National Natural Science Foundation of China[41876164]###208; National Natural Science Foundation of China[41776163]###207; National Natural Science Foundation of China[41776163]; National Natural Science Foundation of China[41876164]; National Natural Science Foundation of China[41376004]; National Natural Science Foundation of China[41406152]; National Key RD Plan[2017YFE0111100-04]; Key Research and Development Plan of Shandong Province[2017GHY215003]; Qingdao Science and Technology Project[17-3-3-21-nsh] |
| WOS研究方向 | Science & Technology - Other Topics |
| WOS类目 | Multidisciplinary Sciences |
| WOS记录号 | WOS:000460123600033 |
| 出版者 | NATURE PUBLISHING GROUP |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/155229 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Yu, Huahua; Li, Pengcheng |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Biol Prod, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, 7 Nanhai Rd, Qingdao 266071, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 通讯作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Yue, Yang,Yu, Huahua,Li, Rongfeng,et al. Insights into individual variations in nematocyst venoms from the giant jellyfish Nemopilema nomurai in the Yellow Sea[J]. SCIENTIFIC REPORTS,2019,9:13. |
| APA | Yue, Yang,Yu, Huahua,Li, Rongfeng,Liu, Song,Xing, Ronge,&Li, Pengcheng.(2019).Insights into individual variations in nematocyst venoms from the giant jellyfish Nemopilema nomurai in the Yellow Sea.SCIENTIFIC REPORTS,9,13. |
| MLA | Yue, Yang,et al."Insights into individual variations in nematocyst venoms from the giant jellyfish Nemopilema nomurai in the Yellow Sea".SCIENTIFIC REPORTS 9(2019):13. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Insights into indivi（3370KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论