The involvement of HSP22 from bay scallop Argopecten irradians in response to heavy metal stress

doi:10.1007/s11033-009-9603-6

IOCAS-IR > 实验海洋生物学重点实验室

	The involvement of HSP22 from bay scallop Argopecten irradians in response to heavy metal stress
	Zhang, Lei 1,2; Wang, Lingling 2; Song, Linsheng 2; Zhao, Jianmin 2; Qiu, Limei 2; Dong, Chaohua 1,2; Li, Fengmei 2; Zhang, Huan 2; Yang, Guanpin 1; Wang, LL, Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
	2010-04-01
发表期刊	MOLECULAR BIOLOGY REPORTS
ISSN	0301-4851
卷号	37 期号:4 页码:1763-1771
文章类型	Article
摘要	Heat shock protein 22 (HSP22) is an important member of small heat shock protein (sHSP) subfamily which plays a key role in the process of protecting cells, facilitating the folding of nascent peptides, and responding to stress. In the present study, the cDNA of HSP22 was cloned from Argopecten irradians (designated as AiHSP22) by rapid amplification cDNA end (RACE) based on the expressed sequence tags (ESTs). The full-length cDNA of AiHSP22 was of 1,112 bp, with an open reading frame of 588 bp encoding a polypeptide of 195 amino acids. The deduced amino acid sequence of AiHSP22 showed high similarity to previously identified HSP22s. The expression patterns of AiHSP22 mRNA in different tissues and in haemocytes of scallops exposed to Cd(2+), Pb(2+) or Cu(2+) were investigated by real-time quantitative RT-PCR. The mRNA of AiHSP22 was constitutively expressed in all examined tissues, including haemocyte, muscle, kidney, gonad, gill and heart. The expression level in heart and muscle was higher than that in other tissues. The mRNA level of AiHSP22 in haemocytes was up-regulated after a 10 days exposure of scallops to Cu(2+), Pb(2+) and Cd(2+). However, the expression of AiHSP22 did not increase linearly along with the rise of heavy metal concentration. Different concentrations of the same metal resulted in different effects on AiHSP22 expression. The sensitive response of AiHSP22 to Cu(2+), Pb(2+) and Cd(2+) stress indicated that it could be developed as an indicator of exposure to heavy metals for the pollution monitoring programs in aquatic environment.; Heat shock protein 22 (HSP22) is an important member of small heat shock protein (sHSP) subfamily which plays a key role in the process of protecting cells, facilitating the folding of nascent peptides, and responding to stress. In the present study, the cDNA of HSP22 was cloned from Argopecten irradians (designated as AiHSP22) by rapid amplification cDNA end (RACE) based on the expressed sequence tags (ESTs). The full-length cDNA of AiHSP22 was of 1,112 bp, with an open reading frame of 588 bp encoding a polypeptide of 195 amino acids. The deduced amino acid sequence of AiHSP22 showed high similarity to previously identified HSP22s. The expression patterns of AiHSP22 mRNA in different tissues and in haemocytes of scallops exposed to Cd2+, Pb2+ or Cu2+ were investigated by real-time quantitative RT-PCR. The mRNA of AiHSP22 was constitutively expressed in all examined tissues, including haemocyte, muscle, kidney, gonad, gill and heart. The expression level in heart and muscle was higher than that in other tissues. The mRNA level of AiHSP22 in haemocytes was up-regulated after a 10 days exposure of scallops to Cu2+, Pb2+ and Cd2+. However, the expression of AiHSP22 did not increase linearly along with the rise of heavy metal concentration. Different concentrations of the same metal resulted in different effects on AiHSP22 expression. The sensitive response of AiHSP22 to Cu2+, Pb2+ and Cd2+ stress indicated that it could be developed as an indicator of exposure to heavy metals for the pollution monitoring programs in aquatic environment.
关键词	Hsp22 Argopecten Irradians Gene Cloning Biomarker Heavy Metals
学科领域	Biochemistry & Molecular Biology
DOI	10.1007/s11033-009-9603-6
收录类别	SCI
语种	英语
WOS记录号	WOS:000275161700012
出版者	SPRINGER
引用统计	被引频次：25[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/145
专题	实验海洋生物学重点实验室
通讯作者	Wang, LL, Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
作者单位	1.Ocean Univ China, Coll Marine Life, Qingdao 266003, Peoples R China 2.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Zhang, Lei,Wang, Lingling,Song, Linsheng,et al. The involvement of HSP22 from bay scallop Argopecten irradians in response to heavy metal stress[J]. MOLECULAR BIOLOGY REPORTS,2010,37(4):1763-1771.
APA	Zhang, Lei.,Wang, Lingling.,Song, Linsheng.,Zhao, Jianmin.,Qiu, Limei.,...&Wang, LL, Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.(2010).The involvement of HSP22 from bay scallop Argopecten irradians in response to heavy metal stress.MOLECULAR BIOLOGY REPORTS,37(4),1763-1771.
MLA	Zhang, Lei,et al."The involvement of HSP22 from bay scallop Argopecten irradians in response to heavy metal stress".MOLECULAR BIOLOGY REPORTS 37.4(2010):1763-1771.

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