The arginine kinase in Zhikong scallop Chlamys farreri is involved in immunomodulation

doi:10.1016/j.dci.2012.03.008

IOCAS-IR > 实验海洋生物学重点实验室

	The arginine kinase in Zhikong scallop Chlamys farreri is involved in immunomodulation
	Shi, Xiaowei 1,2; Wang, Lingling 1; Zhou, Zhi 1,2; Yang, Chuanyan 1,2; Gao, Yang1,2 ; Wang, Leilei 1,2; Song, Linsheng 1; Wang, LL (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China.
	2012-06-01
发表期刊	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN	0145-305X
卷号	37 期号:2 页码:270-278
文章类型	Article
摘要	Arginine kinase (AK) catalyzes the reversible phosphorylation of L-arginine to form phosphoarginine, and plays a critical role in energy metabolism in invertebrates. In the present study, a scallop AK gene was identified from Chlamys farreri with an open reading frame (ORF) of 1101 bp encoding for a protein of 366 amino acids (designed as CfAK). An ATP-gua PtransN domain which was described as a guanidine substrate specificity domain (GS domain) and an ATP-gua Ptrans domian which was responsible for binding ATP, were both identified in CfAK. The mRNA transcripts of CfAK were detectable in haemocytes, hepatopancreas, adductor muscle, mantle, gill, kidney and gonad, with the highest expression level in the muscle and the lowest level in the hemocytes. The expression level of OAK mRNA increased from fertilized eggs to eyebot, and reached the highest in the trochophore stage. The relative expression level of CfAK mRNA in muscle was up-regulated significantly after LPS (0.5 mg/mL) stimulation, and reached the peak at 6 h (5.2-fold, P <0.05). The activity of inducible nitric oxide synthase (iNOS) in the supernatant of muscle homogenate increased significantly from 3.2 U/mg at Oh to 9.7 U/mg at 12 h after LPS stimulation, while the concentration of nitric oxide (NO) in the supernatant of muscle homogenate began to increase at 3 h (21.55 mu mol/L), and reached the top concentration at 24 h (42.27 mu mol/L), then recovered to the normal level after 48 h. The recombinant protein of CfAK (rCfAK) expressed in Escherichia coli displayed Arginine kinase activity, and its apparent K-m was 0.82 +/- 0.11 and 1.24 +/- 0.13 mM for L-arginine and ATP-Na, respectively. The results indicated that the CfAK was involved in energy production and utilization during the whole life process, and might refer to the immunomodulation process via altering the NO concentration and iNOS activity in scallop Chlamys farreri. (C) 2012 Elsevier Ltd. All rights reserved.
关键词	Arginine Kinase (Ak) Immune Response Kinetic Parameters L-arginine Lipopolysaccharide (Lps) Inducible Nitric Oxide Synthase (Inos) Nitric Oxide (No)
学科领域	Immunology ; Zoology
DOI	10.1016/j.dci.2012.03.008
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000306891400006
引用统计	被引频次：30[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/12390
专题	实验海洋生物学重点实验室海洋环境腐蚀与生物污损重点实验室
通讯作者	Wang, LL (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China.
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Shi, Xiaowei,Wang, Lingling,Zhou, Zhi,et al. The arginine kinase in Zhikong scallop Chlamys farreri is involved in immunomodulation[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2012,37(2):270-278.
APA	Shi, Xiaowei.,Wang, Lingling.,Zhou, Zhi.,Yang, Chuanyan.,Gao, Yang.,...&Wang, LL .(2012).The arginine kinase in Zhikong scallop Chlamys farreri is involved in immunomodulation.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,37(2),270-278.
MLA	Shi, Xiaowei,et al."The arginine kinase in Zhikong scallop Chlamys farreri is involved in immunomodulation".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 37.2(2012):270-278.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
The arginine kinase （1087KB）			限制开放	使用许可	浏览