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| Isolation, functional characterization and crystallization of Aq_1259, an outer membrane protein with porin features, from Aquifex aeolicus | |
| Wang, Tao1; Langer, Julian D.1; Peng, Guohong1,2; Michel, Hartmut1; Peng, GH (reprint author), Max Planck Inst Biophys, Dept Mol Membrane Biol, Max von Laue Str 3, D-60438 Frankfurt, Germany. | |
| 2012-12-01 | |
| 发表期刊 | BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
 |
| ISSN | 1570-9639 |
| 卷号 | 1824期号:12页码:1358-1365 |
| 文章类型 | Article |
| 摘要 | The "hypothetical protein" Aq_1259 was identified by mass spectrometry and purified from native membranes of Aquifex aeolicus. It is a 49.4 kDa protein, highly homologous (>52% identity) to several conserved hypothetical proteins from other bacteria. However, none of these proteins has been characterized using biochemical or electrophysiological techniques. Based on the sequence and circular dichroism spectroscopy, the structure of Aq_1259 is predicted to be a beta-barrel with 16 beta-strands. The strands with loops and turns are distributed evenly through the entire sequence. The function of Aq_1259 was analyzed after incorporation into a lipid bilayer. Electrophysiological measurements revealed a pore that has a basic stationary conductance of 0.48 +/- 0.038 nS in a buffer with 0.5 M NaH2PO4 at pH 6.5 and 0.2 +/- 0.015 nS in a buffer with 0.5 M NaCl at pH 6.5. Superimposed on this is a fluctuating conductance of similar amplitude. Aq_1259 could be crystallized. The crystals diffract to a resolution of 3.4 angstrom and belong to space group 1222 with cell dimensions of a = 138.3 angstrom, b = 144.6 angstrom, c = 151.8 angstrom. (C) 2012 Elsevier B.V. All rights reserved. |
| 关键词 | Aq_1259 Porin Aquifex Aeolicus Hypothetical Protein |
| 学科领域 | Biochemistry & Molecular Biology ; Biophysics |
| DOI | 10.1016/j.bbapap.2012.07.004 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000310761700006 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/12356 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Peng, GH (reprint author), Max Planck Inst Biophys, Dept Mol Membrane Biol, Max von Laue Str 3, D-60438 Frankfurt, Germany. |
| 作者单位 | 1.Max Planck Inst Biophys, Dept Mol Membrane Biol, D-60438 Frankfurt, Germany 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Tao,Langer, Julian D.,Peng, Guohong,et al. Isolation, functional characterization and crystallization of Aq_1259, an outer membrane protein with porin features, from Aquifex aeolicus[J]. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS,2012,1824(12):1358-1365. |
| APA | Wang, Tao,Langer, Julian D.,Peng, Guohong,Michel, Hartmut,&Peng, GH .(2012).Isolation, functional characterization and crystallization of Aq_1259, an outer membrane protein with porin features, from Aquifex aeolicus.BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS,1824(12),1358-1365. |
| MLA | Wang, Tao,et al."Isolation, functional characterization and crystallization of Aq_1259, an outer membrane protein with porin features, from Aquifex aeolicus".BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS 1824.12(2012):1358-1365. |
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