Isolation and characterization of Pseudomonas sp DX7 capable of degrading sulfadoxine

doi:10.1007/s10532-011-9522-9

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	Isolation and characterization of Pseudomonas sp DX7 capable of degrading sulfadoxine
	Zhang, Weiwei 1,2,3; Xu, Dongxue4,5 ; Niu, Zongliang 1,2,3; Yin, Kun 1,2,3,5; Liu, Ping 1,2,3; Chen, Lingxin 1,2,3; Chen, LX (reprint author), Chinese Acad Sci, Key Lab Coastal Environm Proc, 17 Chunhui Rd, Yantai 264003, Peoples R China.
	2012-06-01
发表期刊	BIODEGRADATION
ISSN	0923-9820
卷号	23 期号:3 页码:431-439
文章类型	Article
摘要	Given that the intensive application of sulfonamides in aquaculture, animal husbandry and malaria treatment has lead to an increase in sulfonamide discharge into the environment, there is an increasing need to find a way to remediate sulfonamide-contaminated sites. The bacterial strain DX7 was isolated from a marine environment and is capable of degrading sulfadoxine. DX7 was identified as a Pseudomonas sp. based on 16S rRNA gene sequencing. Approximately 30% of sulfadoxine was degraded after Pseudomonas sp. DX7 was inoculated into mineral salt plus tryptone media containing 10 mg l(-1) sulfadoxine for 2 days. The degradation efficiency under different environmental conditions was characterized using HPLC. The optimal temperature and pH for sulfadoxine biodegradation were around 30A degrees C and 6.0, respectively. The optimal concentrations of sulfadoxine and tryptone for sulfadoxine biodegradation were determined to be approximately 30 mg l(-1) and between 2.0 and 8.0 g l(-1), respectively. Cytotoxicity analysis indicated that the metabolites of sulfadoxine generated by Pseudomonas sp. DX7 showed significantly reduced cytotoxicity to Hela cells. These results suggest that Pseudomonas sp. DX7 is a new bacterial resource for degrading sulfadoxine and indicate the potential of the isolated strain in the bioremediation of sulfadoxine-contaminated environments.
关键词	Sulfadoxine Pseudomonas Sp. Degradation Cytotoxicity
学科领域	Biotechnology & Applied Microbiology
DOI	10.1007/s10532-011-9522-9
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000303377300009
引用统计	被引频次：28[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/12355
专题	实验海洋生物学重点实验室海洋生态与环境科学重点实验室
通讯作者	Chen, LX (reprint author), Chinese Acad Sci, Key Lab Coastal Environm Proc, 17 Chunhui Rd, Yantai 264003, Peoples R China.
作者单位	1.Chinese Acad Sci, Key Lab Coastal Environm Proc, Yantai 264003, Peoples R China 2.Chinese Acad Sci, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 5.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Weiwei,Xu, Dongxue,Niu, Zongliang,et al. Isolation and characterization of Pseudomonas sp DX7 capable of degrading sulfadoxine[J]. BIODEGRADATION,2012,23(3):431-439.
APA	Zhang, Weiwei.,Xu, Dongxue.,Niu, Zongliang.,Yin, Kun.,Liu, Ping.,...&Chen, LX .(2012).Isolation and characterization of Pseudomonas sp DX7 capable of degrading sulfadoxine.BIODEGRADATION,23(3),431-439.
MLA	Zhang, Weiwei,et al."Isolation and characterization of Pseudomonas sp DX7 capable of degrading sulfadoxine".BIODEGRADATION 23.3(2012):431-439.

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