Edwardsiella tarda Eta1, an In Vivo-Induced Antigen That Is Involved in Host Infection

doi:10.1128/IAI.00063-12

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	Edwardsiella tarda Eta1, an In Vivo-Induced Antigen That Is Involved in Host Infection
	Sun, Yun 1,3; Zheng, Wen-Jiang 1,2,3; Hu, Yong-Hua 1; Sun, Bo-Guang1 ; Sun, Li 1; Sun, L (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China.
	2012-08-01
发表期刊	INFECTION AND IMMUNITY
ISSN	0019-9567
卷号	80 期号:8 页码:2948-2955
文章类型	Article
摘要	Edwardsiella tarda, a Gram-negative bacterium, is a severe fish pathogen that can also infect humans. In this study, we identified, via in vivo-induced antigen technology, an E. tarda antigen, Eta1, and analyzed its function in a Japanese flounder (Paralichthys olivaceus) model. Eta1 is composed of 226 residues and shares homology with putative bacterial adhesins. Quantitative real-time reverse transcriptase (RT)-PCR analysis indicated that when cultured in vitro, eta1 expression was growth phase dependent and reached maximum at mid-logarithmic phase. During infection of flounder lymphocytes, eta1 expression was drastically increased at the early stage of infection. Compared to the wild type, the eta1-defective mutant, TXeta1, was unaffected in growth but exhibited attenuated overall virulence, reduced tissue dissemination and colonization capacity, and impaired ability to invade flounder lymphocytes and to block the immune response of host cells. The lost virulence of TXeta1 was restored when a functional eta1 gene was reintroduced into the strain. Western blot and immunodetection analyses showed that Eta1 is localized to the outer membrane and exposed on the surface of E. tarda and that recombinant Eta1 (rEta1) was able to interact with flounder lymphocytes. Consistent with these observations, antibody blocking of Eta1 inhibited E. tarda infection at the cellular level. Furthermore, when used as a subunit vaccine, rEta1 induced strong protective immunity in flounder against lethal E. tarda challenge. Taken together, these results indicate that Eta1 is an in vivo-induced antigen that mediates pathogen-host interaction and, as a result, is required for optimal bacterial infection.
关键词	Human Epithelial-cells Iii Secretion System Yersinia-enterocolitica Immune-response Virulence Adhesin Vaccine Strain Fish Identification
学科领域	Immunology ; Infectious Diseases
DOI	10.1128/IAI.00063-12
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000306677300036
引用统计	被引频次：43[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/12315
专题	实验海洋生物学重点实验室
通讯作者	Sun, L (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China.
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Chinese Acad Sci, Natl Sci Lib, Lanzhou Branch, Lanzhou, Gansu, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Sun, Yun,Zheng, Wen-Jiang,Hu, Yong-Hua,et al. Edwardsiella tarda Eta1, an In Vivo-Induced Antigen That Is Involved in Host Infection[J]. INFECTION AND IMMUNITY,2012,80(8):2948-2955.
APA	Sun, Yun,Zheng, Wen-Jiang,Hu, Yong-Hua,Sun, Bo-Guang,Sun, Li,&Sun, L .(2012).Edwardsiella tarda Eta1, an In Vivo-Induced Antigen That Is Involved in Host Infection.INFECTION AND IMMUNITY,80(8),2948-2955.
MLA	Sun, Yun,et al."Edwardsiella tarda Eta1, an In Vivo-Induced Antigen That Is Involved in Host Infection".INFECTION AND IMMUNITY 80.8(2012):2948-2955.

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