| Cytotoxicity of the venom from the nematocysts of jellyfish Cyanea nozakii Kishinouye | |
Li Cuiping2; Li Pengcheng1 ; Feng Jinhua1; Li Rongfeng1; Yu Huahua1; Yu, HH (reprint author), Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
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| 2012-03-01 | |
| 发表期刊 | TOXICOLOGY AND INDUSTRIAL HEALTH
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| ISSN | 0748-2337 |
| 卷号 | 28期号:2页码:186-192 |
| 文章类型 | Article |
| 摘要 | In this article, the cytotoxicity of the venom from the nematocysts of jellyfish Cyanea nozakii Kishinouye on human hepatoma cells (Bel-7402, SMMC-7721) and human colon cancer cells (H630) was investigated first. Of the three kinds of cells, the venom had the strongest cytotoxicity on H630 cells with the 50% lethal concentration (IC50) of 5.1 mu g/ml. However, the IC50 on Bel-7402 and SMMC-7721 was approximate 17.9 and 24.3 mu g/ml, respectively. The cytotoxicity of the venom was affected by pH, temperature and storage conditions. At the pH 4.5-8.5, the venom displayed obvious cytotoxicity and the percentage of survival was about 50%. When pre-incubated at temperatures over 60 degrees C for as short as 10 min, the percentage of survival sharply improved from 4.6% up to 80%. The venom was stored in a more stable condition at -80 degrees C and in lyophilized state compared to other storage conditions used in this study. Lactate dehydrogenase release assay performed on H630 cells indicated that exposure to the venom could result in damage to the cell membrane. |
| 关键词 | Cytotoxicity Jellyfish Nematocysts Venom Cyanea Nozakii Kishinouye |
| 学科领域 | Public, Environmental & Occupational Health ; Toxicology |
| DOI | 10.1177/0748233711410910 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000300222800013 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/12216 |
| 专题 | 海洋生物技术研发中心 海洋环境工程技术研究发展中心 |
| 通讯作者 | Yu, HH (reprint author), Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Ocean Univ China, Qingdao Coll, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li Cuiping,Li Pengcheng,Feng Jinhua,et al. Cytotoxicity of the venom from the nematocysts of jellyfish Cyanea nozakii Kishinouye[J]. TOXICOLOGY AND INDUSTRIAL HEALTH,2012,28(2):186-192. |
| APA | Li Cuiping,Li Pengcheng,Feng Jinhua,Li Rongfeng,Yu Huahua,&Yu, HH .(2012).Cytotoxicity of the venom from the nematocysts of jellyfish Cyanea nozakii Kishinouye.TOXICOLOGY AND INDUSTRIAL HEALTH,28(2),186-192. |
| MLA | Li Cuiping,et al."Cytotoxicity of the venom from the nematocysts of jellyfish Cyanea nozakii Kishinouye".TOXICOLOGY AND INDUSTRIAL HEALTH 28.2(2012):186-192. |
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