Role of MicroRNA-26b in Glioma Development and Its Mediated Regulation on EphA2

doi:10.1371/journal.pone.0016264

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	Role of MicroRNA-26b in Glioma Development and Its Mediated Regulation on EphA2
	Wu, Ning 1,4; Zhao, Xiangzhong 1; Liu, Ming 1; Liu, Haizhou 1; Yao, Weicheng 2; Zhang, Yuyan 1; Cao, Shousong 3; Lin, Xiukun 1
	2011-01-14
发表期刊	PLOS ONE
ISSN	1932-6203
卷号	6 期号:1 页码:e16264
文章类型	Article
摘要	Background: MicroRNAs (miRNAs) are short, non-coding RNAs that regulate the expression of multiple target genes. Deregulation of miRNAs is common in human tumorigenesis. Low level expression of miR-26b has been found in glioma cells. However, its underlying mechanism of action has not been determined.; Background: MicroRNAs (miRNAs) are short, non-coding RNAs that regulate the expression of multiple target genes. Deregulation of miRNAs is common in human tumorigenesis. Low level expression of miR-26b has been found in glioma cells. However, its underlying mechanism of action has not been determined. Methodology/Principal Findings: Real-time PCR was employed to measure the expression level of miR-26b in glioma patients and cells. The level of miR-26b was inversely correlated with the grade of glioma. Ectopic expression of miR-26b inhibited the proliferation, migration and invasion of human glioma cells. A binding site for miR-26b was identified in the 3'UTR of EphA2. Over-expression of miR-26b in glioma cells repressed the endogenous level of EphA2 protein. Vasculogenic mimicry (VM) experiments were performed to further confirm the effects of miR-26b on the regulation of EphA2, and the results showed that miR-26b inhibited the VM processes which regulated by EphA2. Significance: This study demonstrated that miR-26b may act as a tumor suppressor in glioma and it directly regulates EphA2 expression. EphA2 is a direct target of miR-26b, and the down-regulation of EphA2 mediated by miR-26b is dependent on the binding of miR-26b to a specific response element of microRNA in the 3'UTR region of EphA2 mRNA.
学科领域	Life Sciences & Biomedicine - Other Topics
DOI	10.1371/journal.pone.0016264
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000286516500042
引用统计	被引频次：111[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/11931
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China 2.Qingdao Med Univ, Inst Brain, Affiliated Hosp, Qingdao, Peoples R China 3.Roswell Pk Canc Inst, Buffalo, NY 14263 USA 4.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Wu, Ning,Zhao, Xiangzhong,Liu, Ming,et al. Role of MicroRNA-26b in Glioma Development and Its Mediated Regulation on EphA2[J]. PLOS ONE,2011,6(1):e16264.
APA	Wu, Ning.,Zhao, Xiangzhong.,Liu, Ming.,Liu, Haizhou.,Yao, Weicheng.,...&Lin, Xiukun.(2011).Role of MicroRNA-26b in Glioma Development and Its Mediated Regulation on EphA2.PLOS ONE,6(1),e16264.
MLA	Wu, Ning,et al."Role of MicroRNA-26b in Glioma Development and Its Mediated Regulation on EphA2".PLOS ONE 6.1(2011):e16264.

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