Detection of type III secretion gene as an indicator for pathogenic Edwardsiella tarda

doi:10.1111/j.1472-765X.2010.02984.x

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	Detection of type III secretion gene as an indicator for pathogenic Edwardsiella tarda
	Li, G. Y.1,2; Li, J.1,2 ; Xiao, P.1; Guo, Y. H.3; Mo, Z. L.1
	2011-03-01
发表期刊	LETTERS IN APPLIED MICROBIOLOGY
ISSN	0266-8254
卷号	52 期号:3 页码:213-219
文章类型	Article
摘要	Aims:; Aims: To differentiate pathogenic and nonpathogenic Edwardsiella tarda strains based on the detection of type III secretion system (T3SS) gene using polymerase chain reaction (PCR). Methods and Results: Primers were designed to amplify Edw. tarda T3SS component gene esaV, catalase gene katB, haemolysin gene hlyA and 16S rRNA gene as an internal positive control. Genomic DNAs were extracted using a commercial isolation kit from 36 Edw. tarda strains consisting of 18 pathogenic and 18 nonpathogenic strains, and 50 ng of each DNA was used as the template for PCR amplification. PCR was performed with a thermocycler (TaKaRa TP600) in a 25-mu l volume. Products of esaV were detected in all pathogenic strains, but not in nonpathogenic strains; katB was detected in all pathogenic strains and one of nonpathogenic strains; hlyA was not detected in any strains. Conclusions: The detection of esaV gene can be used for the assessment of pathogenic Edw. tarda strains. Significance and Impact of the Study: The strategy using T3SS gene as the virulence indicator provides a useful tool for the clinical assessment of pathogenic Edw. tarda strains and prediction of edwardsiellosis risk in fish culture environments.
关键词	Edwardsiella Tarda Pathogen Detection T3ss
学科领域	Biotechnology & Applied Microbiology ; Microbiology
DOI	10.1111/j.1472-765X.2010.02984.x
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000287038400005
引用统计	被引频次：16[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/11829
专题	实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China 3.Fujian Key Lab Med Instrument & Pharmaceut Techno, Fuzhou, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Li, G. Y.,Li, J.,Xiao, P.,et al. Detection of type III secretion gene as an indicator for pathogenic Edwardsiella tarda[J]. LETTERS IN APPLIED MICROBIOLOGY,2011,52(3):213-219.
APA	Li, G. Y.,Li, J.,Xiao, P.,Guo, Y. H.,&Mo, Z. L..(2011).Detection of type III secretion gene as an indicator for pathogenic Edwardsiella tarda.LETTERS IN APPLIED MICROBIOLOGY,52(3),213-219.
MLA	Li, G. Y.,et al."Detection of type III secretion gene as an indicator for pathogenic Edwardsiella tarda".LETTERS IN APPLIED MICROBIOLOGY 52.3(2011):213-219.

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