Institutional Repository of Key Laboratory of Marine Ecology & Environmental Sciences, CAS
| Cloning and nucleotide sequence of D-hydantoinase gene of marine polyphosphate-accumulating bacterium, Halomonas sp YSR-3 | |
| Ren Shiying2; Li Xiangqian2; Jia Jianbo2; Liu Fei2; Xiao Tian1 | |
| 2011-09-01 | |
| 发表期刊 | CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
 |
| ISSN | 0254-4059 |
| 卷号 | 29期号:5页码:917-921 |
| 文章类型 | Article |
| 摘要 | Hydantoinase is involved in the production of optically pure amino acids from racemic 5-mono-substituted hydantoins. We measured the D-hydantoinase activity in marine Halomonas sp. YSR-3 and amplified the D-hydantoinase gene by PCR. The gene was inserted into vector pGM-T and transformed into E. coli TOP10. The positive transformants with the D-hydantoinase gene were sequenced. The sequenced fragment comprises 1 510 base pairs. The D-hydantoinase gene from YSR-3 is 77% similar to that from Pseudomonas entomophila L4 by searching against the NCBI databse. The protein product of the YSR-3 D-hydantoinase gene is 75%, 73%, and 70% similar to those from Pseudomonas fluorescens Pf-5, Marinomonas sp. MED121, and Burkholderia vietnamiensis G4, respectively. The difference of the D-hydantoinase gene between marine Halomonas sp. YSR-3 and other terrestrial organisms is distinct.; Hydantoinase is involved in the production of optically pure amino acids from racemic 5-mono-substituted hydantoins. We measured the D-hydantoinase activity in marine Halomonas sp. YSR-3 and amplified the D-hydantoinase gene by PCR. The gene was inserted into vector pGM-T and transformed into E. coli TOP10. The positive transformants with the D-hydantoinase gene were sequenced. The sequenced fragment comprises 1 510 base pairs. The D-hydantoinase gene from YSR-3 is 77% similar to that from Pseudomonas entomophila L4 by searching against the NCBI databse. The protein product of the YSR-3 D-hydantoinase gene is 75%, 73%, and 70% similar to those from Pseudomonas fluorescens Pf-5, Marinomonas sp. MED121, and Burkholderia vietnamiensis G4, respectively. The difference of the D-hydantoinase gene between marine Halomonas sp. YSR-3 and other terrestrial organisms is distinct. |
| 关键词 | Halomonas Sp Ysr-3 D-hydantoinase Polyphosphate-accumulating Bacterium |
| 学科领域 | Marine & Freshwater Biology ; Oceanography |
| DOI | 10.1007/s00343-011-0071-1 |
| URL | 查看原文 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000293632500001 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/11649 |
| 专题 | 海洋生态与环境科学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Huaiyin Inst Technol, Sch Life Sci & Chem Engn, Huaian 223003, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ren Shiying,Li Xiangqian,Jia Jianbo,et al. Cloning and nucleotide sequence of D-hydantoinase gene of marine polyphosphate-accumulating bacterium, Halomonas sp YSR-3[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2011,29(5):917-921. |
| APA | Ren Shiying,Li Xiangqian,Jia Jianbo,Liu Fei,&Xiao Tian.(2011).Cloning and nucleotide sequence of D-hydantoinase gene of marine polyphosphate-accumulating bacterium, Halomonas sp YSR-3.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,29(5),917-921. |
| MLA | Ren Shiying,et al."Cloning and nucleotide sequence of D-hydantoinase gene of marine polyphosphate-accumulating bacterium, Halomonas sp YSR-3".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 29.5(2011):917-921. |
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